Caspase-3与β-catenin在分化型甲状腺癌中的表达及意义  

Expression of caspase-3 and β-catenin in differentiated thyroid cancer and its significance

作　　者：贾金良[1] 赵雪松[1] 鲁金乐[1] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院头颈外科,哈尔滨150040

出　　处：《临床肿瘤学杂志》2009年第9期782-786,共5页Chinese Clinical Oncology

基　　金：黑龙江省卫生厅2006年资助项目(C2006192)

摘　　要：目的:研究Caspase-3和β-catenin蛋白在分化型甲状腺癌(DTC)中的蛋白表达情况,探讨它们在DTC发生发展中的作用及相互关系。方法:采用免疫组织化学SP法,对52例DTC组织和10例正常甲状腺组织中的Caspase-3和β-cate-nin蛋白表达情况进行检测。结果:DTC组织中的Caspase-3蛋白表达率为51.92%(27/52),明显低于正常甲状腺组织的90.00%(9/10),Caspase-3蛋白的表达与DTC病理分级有关(P<0.05),但与临床分期及颈淋巴结转移无关;DTC组织中的β-catenin蛋白表达率为67.31%(35/52),明显高于正常甲状腺组织的20.00%(2/10),β-catenin蛋白的表达与DTC颈淋巴结转移有关(P<0.05),但与临床分期及病理分级无关;在DTC组织中Caspase-3和β-catenin蛋白的表达呈负相关(rs=-0.659,P<0.01)。结论:Caspase-3和β-catenin蛋白的异常表达共同参与了分化型甲状腺癌的发生发展,可能成为判断分化型甲状腺癌恶性程度、转移及预后的指标。Objective：This study investigated the expression of caspase-3 and β-catenin in differentiated thyroid cancer （DTC） tissues,approached therole and correlation of caspase-3 and β-catenin in the carcinogenesis of the thyroid. Methods：The im- munohistocemistry （ SP method）was used to determine the expression of caspase-3 and β-catenin in 52 cases of DTC and 10 normal thy- roid tissues. Results：The protein expression level of caspase-3 in DTC 51.92% （27/52） was significantly lower than that of normal thyroid sample 90. 00% （9/10）. Caspase-3 expression level was correlated with the grade of DTC （ P 〈 0.05 ）, but had no significant correlation with clinical stages and cervical lymph node metastasis. The protein expression level of β-catenin in DTC 67. 31% （35/52） was significantly higher than that in normal thyroid sample 20. 00% （2/10）. β-catenin expression level was correlated with cervical lymph node metastasis （P 〈 0.05 ）, but had no significant correlation with clinical stages and the grade of DTC. Conclusion：The abnormal expression of caspase-3 and β-catenin may have an important role in the carcinogenesis of the thyroid, and they probably have a limited role in malignant progression of the thyroid cancer. They can was used as the possible marker which judge the malignancy degree,metastasis and prognosis of thyroid cancer.

关 键 词：CASPASE-3 Β-CATENIN 分化型甲状腺癌 免疫组织化学 

分 类 号：R736.1[医药卫生—肿瘤]