COX-2和MMP-9在食管鳞癌组织中的表达及其与血管生成的关系  

Expression of cyclooxygenase-2 and matrix metalloproteinase-9 in human esophageal squamous cell carcinoma and its correlation with angiogenesis

作　　者：产松苗 欧希龙[2] 孙为豪[3] 陈国胜[2] 颜芳[2] 

机构地区：[1]上海梅山医院消化科,江苏省南京市210039 [2]东南大学临床医学院附属中大医院消化科,江苏省南京市210009 [3]南京医科大学第一附属医院老年医学科,江苏省南京市210029

出　　处：《世界华人消化杂志》2009年第22期2259-2265,共7页World Chinese Journal of Digestology

摘　　要：目的:观察食管鳞癌组织、癌旁正常黏膜中环氧化酶-2(COX-2)及基质金属蛋白酶-9(MMP-9)的表达及其与微血管密度(MVD)的关系,探讨COX-2和MMP-9对食管鳞癌血管生成的作用与意义.方法:应用SP法对90例食管鳞癌组织和34例癌旁正常黏膜的COX-2、MMP-9及CD34进行免疫组织化学染色,检测癌组织、癌旁正常食管黏膜组织的COX-2与MMP-9表达及MVD,并分析COX-2、MMP-9的表达与MVD之间,以及他们与食管鳞癌临床病理特征之间的关系.结果:食管鳞癌组织中COX-2、MMP-9的阳性表达率和MVD分别为84.8%、82.2%和29.70±3.82,显著高于癌旁正常黏膜的20.6%、14.7%和15.1±2.38.COX-2的表达与肿瘤的TNM分期、分化程度和淋巴结转移密切相关(P<0.01).MMP-9、MVD的表达与肿瘤的TNM分期和淋巴结转移密切相关(P<0.01);COX-2表达与MVD呈显著正相关(r=0.607,P<0.01);COX-2与MMP-9的表达正相关(r=0.740,P<0.01);MMP-9的表达与MVD值之间呈正相关(r=0.718,P<0.01).结论:COX-2与MMP-9异常表达在食管鳞癌的血管生成中起重要作用,COX-2、MMP-9及其诱导的血管生成与食管鳞癌的侵袭和转移密切相关.AIM： To investigate the expression of COX-2 and MMP-9 in human esophageal squamous cell carcinoma and explore its correlation with angiogenesis. METHODS： Ninety tumor specimens from patients with esophageal squamous cell carcinoma were obtained, and 34 corresponding paracan- cerous normal tissues were randomly selected as controls. Immunohistochemical staining was used for detecting the expression of COX-2 and MMP-9. Monoclonal antibody against CD34 was used for displaying vascular endothelial cells, and microvascular density （MVD） was deter- mined by counting of CD34-positive vascular endothelial cells.RESULTS： The positive expression rates of COX-2 and MMP-9 and MVD in esophageal squamous cell carcinoma were significantly higher than those in the normal esophageal mucosa（84.8% vs 20.6%, 82.2% vs 14.7% and 29.70± 3.82 vs 15.10±2.38, respectively; all P 〈0.01）. The expression of COX-2 was closely related to UICC-AJCC TMN stage, cellular differentiation and lymph node metastasis （all P 〈 0.01）, while the expression of MMP-9 and MVD was closely related to UICC-AJCC TMN stage and lymph node metastasis （both P 〈 0.01）. COX-2 expression was positively correlated to MVD （r = 0.607, P 〈 0.01） and MMP-9 expression （r = 0.740, P 〈 0.01）, while MMP-9 expression was also posi- tively correlated to MVD （r = 0.718, P 〈 0.01）. CONCLUSION： Both COX-2 and MMP-9 play an important role in the angiogenesis of esopha- geal squamous cell carcinoma. The expression of COX-2 and MMP-9 and tumor neovasculariza- tion are closely related to tumor invasion and lymph node metastasis.

关 键 词：食管鳞癌 环氧化酶-2 基质金属蛋白酶-9 血管生成 免疫组织化学 

分 类 号：R735.1[医药卫生—肿瘤]