直肠癌中肿瘤相关巨噬细胞、基质金属蛋白酶-9与微淋巴管生成关系  

The Relationship among Tumor-associated Macrophages、 Matrix Metalloproteinase-9 and Lymphangiogenes in Rectal Carcinoma

作　　者：詹庆华[1] 陈维荣[1] 蔡高阳[1] 陈业晞[1] 廖梓群[1] 林广荣[1] 林凯煌[1] 

机构地区：[1]汕头大学医学院第二附属医院普外科,广东汕头515041

出　　处：《汕头大学医学院学报》2009年第3期145-149,F0004,共6页Journal of Shantou University Medical College

摘　　要：目的:研究直肠癌的微淋巴管生成情况、肿瘤相关巨噬细胞(TAM)计数与基质金属蛋白酶(MMP)-9表达的关系,探讨TAM与MMP-9在直肠癌微淋巴管生成中的作用。方法:通过免疫组化法测定41例直肠癌标本微淋巴管密度(LMVD)、TAM密度及MMP-9的表达,并分析LMVD、MMP-9与直肠癌其它临床病理学因素间的关系。结果:①直肠癌组织LMVD、TAM密度、MMP-9的表达明显高于正常组织(P<0.05)。②TAM浸润与MMP-9表达和肿瘤微淋巴管生成相关(P<0.05)。③三者与肿瘤分化程度、Dukes分期、淋巴浸润和淋巴结转移等有不同程度的相关性。结论:TAM与MMP-9、直肠癌微淋巴管生成在直肠癌转移中起着重要的作用,MMP-9很可能是肿瘤微淋巴管生成的始动因子之一。Objective： To investigate the relationship among lymphangiogenes, tumor-associated maerophages（TAM） and matrix metalloproteinase-9（MMP-9）in rectal carcinoma, and to discuss the pivotal roles of TAM and MMP-9 in lymphangiogenes of rectal carcinoma. Methods： Forty-one resected tumor specimens were obtained from patients and the immunohistochemical staining was used for detecting the expression of MMP-9, lymphatic microvessel density（LMVD） and TAM density, and the statistics was used for analyzing the inter-relationships and the correlation among them and clinicopathological parameters. Results： LMVD, TAM density and the expression of MMP-9 in rectal carcinoma were significantly higher than those in normal tissues（ P 〈 0.05）; Correlations were found among TAM invasion, the expression of MMP-9 and the lymphangiogenes（ P 〈 0.05）; LMVD, TAM density and the expression of MMP-9 correlated with histologic type, infiltration extent（Dukes）, lymphatic invasion, lymph node metastasis at different degrees. Conclusion： TAM density, the expression of MMP-9 and lymphangiogenes may be critical in rectal carcinoma metastasis. MMP-9 supposes to be one of the initiating factors of carcinoma lymphangiogenes.

关 键 词：直肠癌 肿瘤相关巨噬细胞 基质金属蛋白酶-9 微淋巴管生成 

分 类 号：R735.37[医药卫生—肿瘤]