低磷奶牛血红蛋白尿症红细胞膜脂质过氧化损伤的观察  

Observation on the peroxide-caused plasma membrane lipid damage in the erythrocytes of the phosphorus-deficient dairy cows with hemoglobinura

作　　者：陈维多[1] 王燕[1] 吴明福[1] 徐世文[1] 

机构地区：[1]东北农业大学

出　　处：《黑龙江畜牧兽医》1998年第9期1-2,共2页Heilongjiang Animal Science And veterinary Medicine

基　　金：黑龙江省自然科学基金

摘　　要：研究观察了土壤、牧草低磷条件下，诱发奶牛红细胞膜脂质过氧化损伤。发现血清磷、红细胞膜总脂和过氧化物歧化酶（ＳＯＤ）明显下降：血清磷由（４３．１０±８．１０３）ｍｇ／ｇ下降至（２１．３３±２．２３１）ｍｇ／ｇ；膜总脂由（０．００２６７±０．０００１２９）ｇ／ｍＬ下降至（０．００２２４±０．０００１２９）ｇ／ｍＬ；ＳＯＤ由（２１７．６５±３．０４１）Ｕ／ｍＬ下降至（４０．６２±５．４４）Ｕ／ｍＬ，差异显著（Ｐ＜０．０１）。但微粘度（η）和红细胞丙二醛（ＭＤＡ）上升：η值由０．８４３３±０．０２１５上升至１．７２８５±０．１４５１，表明膜流动性下降；ＭＤＡ由（０．５３６±０．０７８）ｎｍｏｌ／ｍＬ上升至（１．０９３±０．１８０）ｎｍｏｌ／ｍＬ，差异显著（Ｐ＜０．０１）。结果显示，低磷不仅引起奶牛血红蛋白尿症而且诱导红细胞膜脂质过氧化损伤。The problem whether the low phosporus content in the soil andforage grass could cause peroxide-related damage in the membrane lipid of the erythrocyte was investigated in the cow.It was found that thecontent of phosphorus in the serum,the total content of lipid in theerythrocyte membrane and the superoxide dismutase(SOD)declined markedly(P<0.01).The extent of decrease for the content of phosphorus in theserum was from 43.1±8.1 mg to (21.3±2.2) mg the extent for the total content of lipid in the erythrocyte membrane was from (0.0027±0.00013) g/mL to (0.0022±0.00013) g/mL and that for the SOD was from (217.65±3.041) U/mL to (40.62±5.44) U/mL. The microviscosity (n)and the MDA,however, increased. The n value increased from 0.843±0.0215 to 1.729±0.145, indicating a decreased membrane fluidity. The MDA increased from (0.536±0.078) nmol/mL to (1.093±0.18) nmol/mL and the difference was significant (P<0.01).These results suggested that the deficiency of phosporus in the cow caused not only the hemoglobinura but also the peroxide-related damage of the membrane lipid in the erythrocyte.

关 键 词：奶牛 低磷 血红蛋白尿 红细胞膜 损伤 

分 类 号：S858.237.1[农业科学—临床兽医学]