清肠栓对大鼠溃疡性结肠炎白细胞介素受体表达的影响  

Experimental studies on the effects of interleukin receptors expressions in model rats with ulcerative colitis by Qing-chang suppository

作　　者：陈江[1] 马贵同[2] 谢建群[2] 唐志鹏[2] 龚雨萍[2] 张涛[2] 

机构地区：[1]苏州市中医院消化科,江苏苏州215003 [2]上海中医药大学附属龙华医院消化科,上海200032

出　　处：《中国中西医结合消化杂志》2009年第5期309-312,共4页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

摘　　要：[目的]从白细胞介素受体(IL-R)角度探讨中药清肠栓防治溃疡性结肠炎(UC)的作用机制。[方法]三硝基苯磺酸(TNBS)诱导建立大鼠UC模型;41只雄性SD大鼠随机分成6组(正常组,模型组,5-氨基水杨酸栓组,清肠栓大剂量、中剂量、小剂量组),治疗组均分别予肠道给药;1周后处死,ELISA法检测血清、结肠组织可溶性IL-R(sIL-R)2、4、6水平;RT-PCR半定量法检测结肠黏膜IL-2RI、L-4RI、L-6R mRNA的表达。[结果]清肠栓各剂量组sIL-2R、sIL-6R的水平降低,与模型组比较P<0.01;中、大剂量组IL-2RI、L-6R mRNA表达降低,与模型组比较P<0.01或<0.05;清肠栓各剂量组皆有降低IL-4R mRNA表达的趋势,与模型组比较P>0.05。[结论]清肠栓有效防治UC,其作用机制与降低sIL-2R、sIL-6R水平,下调IL-2RI、L-6R mRNA表达,抑制T淋巴细胞活化增殖,减少炎症递质产生有关。[Objective]To investigate the mechanism of curative effect on UC by Qing-chang suppository from the angle of interleukin receptor expressions. [Methods]Rat models with ulcerative colitis induced by a solution containing trinitrobenzene sulfonic acid and ethanol were established. All forty-one SD rats were randomly divided into six groups（nomal control, model control, 5-ASA suppository, three different dose of Qing-chang suppository groups）. The latter four groups were administered by enema once a day for a week. Soluble interleukin receptors 2,4, 6 both in serum and in colon tissue were examined by ELISA, and mRNA expressions of interleukin receptor 2,4,6 by RT-PCR. [Results]The levels of sIL-2R, sIL-6R in each Qing-chang suppository group markedly reduced, and mRNA expression of interleukin receptor 2,6 in high and medium dose group decreased, P〈0. 01 or P〈0. 05 comparison with those of model group; Each qingchang suppository group presented descending trends of interleukin 4 receptor mRNA expression, but P〈0. 05 comparison with model group. [Conclusion]Qing-chang suppository has curative effects on UC,one of its mechanism may be in relation to reducing the level of soluble interleukin receptors 2, 6, downregulating mRNA of interleukin receptors 2, 6 effetively, inhibiting T cells ＇proliferation and reducing inflammation mediators.

关 键 词：清肠栓 结肠炎 溃疡性 白细胞介素受体 

分 类 号：R574.62[医药卫生—消化系统]