荧光定量PCR法检测鼻咽癌患者全血和血浆标本EB病毒DNA的比较研究  

Comparison of Whole Blood and Plasma for Detection and Quantification of Epstein-Barr Virus DNA in Nasopharyngeal Carcinoma Patients by Fluorescent Quantitative PCR

作　　者：刘涤瑕[1] 王丽[1] 董凤珍[1] 徐立风[1] 唐德羽[1] 陈金超[1] 

机构地区：[1]中国人民解放军第532医院检验科,安徽黄山245041

出　　处：《现代检验医学杂志》2009年第5期93-95,共3页Journal of Modern Laboratory Medicine

摘　　要：目的研究全血和血浆标本对荧光定量PCR方法检测鼻咽癌患者EB病毒DNA的差异。方法收集40例鼻咽癌患者的全血和血浆标本，用荧光定量PCR方法检测其EB病毒DNA含量，并对结果进行比较分析。结果全血和血浆标本的EBV—DNA阳性率（含弱阳性）分别为85．0％和72．5％，差异无统计学意义（χ^2=3．20，P〉0．05），但不含弱阳性则阳性率分别为62．5％和32．5％，差异有统计学显著性意义（χ^2=10．08，P〈0．01）；全血和血浆标本EB病毒DNA浓度几何平均浓度分别为3．58±1．42和4．72±1．53log copies／ml，差异有统计学显著性意义（t=10．48，P〈0．001）；全血和血浆标本EB病毒DNA浓度有一定相关性（r^2=0．787），但97．5％的患者全血载量高于血浆载量。结论荧光定量PCR法检测EB病毒DNA全血标本好于血浆标本，更适合在临床实验室应用。Objective To investigate Epstein-Barr virus DNA loads in whole blood and plasma. Methods Using fluorescent quantitative PCR （FQ-PCR） to evaluate EBV DNA in whole blood and plasma in 40 samples from nasopharyngeal carcinoma patients,and study correlation between the two sample types. Results The ratio of positive of whole blood and plasma were respectively 85.0% and 72.50/6 ,no significant difference （χ^2 = 3.20,P〉0. 05）. The ratio of positive （except weakly positive ） of whole blood and plasma were respectively 62. 5% and 32. 5%,high significant difference （χ^2= 10. 08,P〈 0. 01 ）. The samples of whole blood and plasma concentration of EB virus DNA loads mean cancentrations were 3.58 ± 1.42 log copies/ml and 4.72 ±1.53 log copies/ml,were significantly different （χ^2 = 10.48,P〈0. 001）. The whole blood and the plasma loads correlated moderately （r^2=0. 787,P〈0. 001）;97.5% of patients whose EBV DNA loads in the whole blood were higher than that in plasma,and the average EBV DNA loads in the whole blood were 5 times of that in plasma. Conclusion The whole blood specimen is more efficiently than the plasma specimen,and it is the preferred specimen in diagnostic setting and monitoring EBV-associated diseases.

关 键 词：EB病毒DNA 荧光定量PCR 全血 血浆 鼻咽癌 

分 类 号：R739.63[医药卫生—肿瘤] R730.45[医药卫生—临床医学]