原发性胆汁性肝硬化患者血清抗线粒体抗体M2亚型定量检测的临床价值  

Clinical Value of Quantitative Determination of Anti-mitochondrial Antibody Type 2（M2） in Serum of Patients with Primary Biliary Cirrhosis

作　　者：杨晏[1] 李维[1] 王忠诚[1] 

机构地区：[1]重庆市急救医疗中心检验科,重庆400014

出　　处：《现代检验医学杂志》2009年第5期98-100,共3页Journal of Modern Laboratory Medicine

摘　　要：目的研究抗线粒体抗体M2亚型（AMA—M2）定量检测对原发性胆汁性肝硬化（PBC）诊断的临床价值。方法采用酶联免疫吸附试验（ELISA）检测20例健康人、21例PBC患者及56例其他患者的血清AMA—M2水平，应用矩阵决策法分析AMA—M2检测对PBC的诊断效率，并分析比较其与传统免疫印迹法检测结果的一致性。结果ELISA法检测血清AMA—M2的线性回归方程为LogY=1．3517△A＋1．1419，r=0．998，线性可达800RU／ml。与传统免疫印迹法相比较，Kappa值为0．87。健康人血清AMA—M2水平为（17．10±1．80）RU／ml；其他疾病患者中病毒性肝炎为（20．40±5．17）RU／ml，胆道疾病（23．00±11．54）RU／ml，SLE（18．24±3．36）RU／m1．其他自身免疫性疾病（19．80±9．39）RU／ml，分别与健康人群相比较差异均无统计学意义（P〉0．05）。PBC患者血清AMA—M2水平为（207．40±174．90）RU／ml，显著高于健康人群（P〈0．01）。AMA—M2检测对PBC诊断的敏感度为95．2％．特异度96．1％，诊断符合率95．9％，阳性预测值87．0％，阴性预测值98．6％。结论ELISA法定量检测血清AMA—M。操作简便，结果准确可靠，诊断效率高，可作为PBC诊断的重要免疫学指标。Objective To study the clinical value of quantitative determination of anti-mitochondrial antibody type 2（AMA- M2） for diagnostic of primary biliary cirrhosis （PBC）. Methods AMA-M2 concentration was determined in 20 healthy subjects, 21 PBC patients and another 56 patients with other diseases by enzyme-linked immunosorbent assay （ELISA）,and the diagnose efficiency managed matrix decision method. To analyze and compare the result consistency with conventional western blot（WB）. Results The regression analysis of AMA-M2 determined with ELISA method showed a good correlation between the logY and △A （logY = 1. 351 7△A＋1. 141 9, r = 0. 998）, and the detection linearity was up to 800 RU/ ml. Comparing with conventional WB method, the Kappa value was 0. 87. AMA-M2 concentration were （17.10± 1.80） RLl/ml in the serum of healthy subjects, （20. 40 ± 5.17）RU/mt, （23.00 ± 11.54）RU/ml, （18.24±3.36）RU/ml and （19.80±9.39）RU/ml in other patients with viral hepatitis,biliary tract diseases,SLE and other autoimmune diseases,respectively. There were no statistical difference compared to healthy subjects （P〉0. 05）.AMA-M2 concentration was （207.40±174. 90）RU/ml in the serum of patients with PBC,the result was much higher than the healthy subjects （P〈 0. 01）. The sensitivity,specificity ,accuracy ,positive predictive rate and negative predictive rate of AMA-M2 determined for laboratory diagnosis of PBC were respectively 95.2% ,96.1% ,95.9% ,87.0% and 98.6% respectively. Conclusion The quantitative determination of AMA-M2 in the serum by ELISA method is easy to perform,reliable and much higher on diagnose efficiency. AMA-M2 detection is an important immunological marker for the diagnosis of PBC.

关 键 词：原发性胆汁性肝硬化 抗线粒体抗体M2亚型 酶联免疫吸附试验 

分 类 号：R575.22[医药卫生—消化系统] R392.11[医药卫生—内科学]