采用Raman光谱研究小麦胚芽8S球蛋白的二级结构  被引量:4

Raman Spectroscopic Study of Secondary Structure of Wheat Germ 8S Globulin

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作  者:朱科学[1] 郭晓娜[1] 彭伟[1] 周惠明[1] 

机构地区:[1]江南大学食品学院,江苏无锡214122

出  处:《食品科学》2009年第19期32-34,共3页Food Science

基  金:江苏省自然科学基金项目(BK2008102);高等学校博士学科点专项科研基金项目(20070295034)

摘  要:采用显微激光拉曼光谱仪研究了小麦胚芽8S球蛋白的二级结构。结果表明:小麦胚芽8S球蛋白的二级结构主要为β折叠,另外还含有一定量的α螺旋和无规卷曲构象,小麦胚芽8S球蛋白的C—C—S—S—C—C侧链为扭曲—扭曲—扭曲式和扭曲—扭曲—反式两种构型,其酪氨酸残基趋于全部暴露于溶剂中,而其色氨酸残基趋向于埋藏。Wheat germ 8S globulin is one of the most important components of globulins. Further investigation of wheat germ 8S globulin structure information will be helpful for exploitation and utilization of wheat germ resource. In this study, the secondary structure of wheat germ 8S globulin was determined using Raman spectroscopy. Results indicated that β -sheets followed by a-helical structure were the major component of wheat germ 8S globulin and some random coil structures were also observed in it. C-C-S-S-C-C side chain in this globulin revealed as the transfigurations of gauche-gauche-gauche and gauche-gauche-trans. All tyrosyl residues were exposed completely, while Trp residues were buried inside.

关 键 词:小麦胚芽 8S球蛋白 拉曼光谱 二级结构 

分 类 号:R284.1[医药卫生—中药学]

 

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