胃癌p16基因启动子CpG岛甲基化研究  

Hypermethylation of CpG island at p16 gene promoter in gastric carcinomas

作　　者：罗冲[1] 周永宁[1] 郭娴[1] 杨永成[1] 

机构地区：[1]兰州大学第一医院消化内科,甘肃兰州730000

出　　处：《第四军医大学学报》2009年第18期1659-1661,共3页Journal of the Fourth Military Medical University

基　　金：中科院西部之光项目(中科院文件2005第404号)

摘　　要：目的:探讨p16基因启动子CpG岛甲基化在胃癌中的表达及意义.方法:采用特异性甲基化PCR(MSP)法检测p16基因启动子CpG岛甲基化水平.结果:对照组未检测到CpG岛甲基化,胃癌细胞株均检测到CpG岛甲基化,23例(74%)胃癌组织检测到CpG岛甲基化.此外,17例(74%)癌旁组织检测到CpG岛甲基化.胃癌组织p16基因CpG岛甲基化与分化程度及淋巴结转移之间无相关关系(P>0.05).结论:胃癌广泛存在p16基因启动子CpG岛甲基化,并且可能是胃癌发生的早期分子事件.AIM： To investigate the expression of hypermethylation of CpG island at the p16 gene promoter in gastric carcinomas and its significance. METHODS： Hypermethylation of CpG island at the p16 gene promoter was determined by methylationspecific polymerase chain reaction （MSP） analysis. RESULTS： CpG-island hypermethylaion was not detected in control biopsy specimens but was identified in and 74. 1% （ 23/31 ） of gastric human gastric cancer cell lines carcinoma patients. CpG-island hypermethylaion of p16 gene was detected in 17 adjacent gastric tissues （73.9% , 17/23）. In primary gastric tumors, no significant association was found between tumor differentiation status and lymph node metastasis and CpG island methylation status of p16 gene （P 〉 0.05, respectively）. CONCLUSION： Hypermethylation of CpG island at the p16 gene promoter is detected commonly in gastric carcinoma, p16 promoter hypermethylation may be involved in the early gastric carcinogenesis process.

关 键 词：P16 胃癌 甲基化 

分 类 号：R735.2[医药卫生—肿瘤]