磷酸化壳聚糖膜引导牙周组织再生的实验研究  

An experimental study of phosphonized chitosan membrane in guiding tissue regeneration

作　　者：王亚红[1] 张文静[1] 李全利[1] 蒋勇[1] 

机构地区：[1]安徽医科大学口腔医学院,合肥230032

出　　处：《安徽医科大学学报》2009年第5期573-576,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省教育厅自然科学基金(编号:2006kj310B);安徽省自然科学基金(编号:070413145);国家自然科学基金(编号:30772440)

摘　　要：目的评价磷酸化壳聚糖膜引导牙周组织再生的效果。方法选择Beagle犬3条,采用丝线结扎及高糖饮食的方法制备慢性牙周炎模型,随机将所制备的24颗牙周炎实验牙分为一个对照组和两个实验组。A实验组使用磷酸化壳聚糖膜,B实验组放置载药的磷酸化壳聚糖膜,C组仅给予翻瓣术作为对照组。术后12周,病理切片组织学观察,统计学分析相关数据。结果动物实验发现新生牙槽骨、牙骨质和结缔组织附着分别为:A实验组:(1.22±0.15)mm、(1.00±0.15)mm、(1.72±0.17)mm,B实验组:(1.44±0.14)mm、(1.20±0.19)mm、(2.27±0.18)mm,C空白对照组:(0.56±0.16)mm、(0.51±0.14)mm、(1.27±0.16)mm。A组和B组牙周组织修复再生的效果好于C组,B组好于A组,差异具有统计学意义(P<0.05)。结论磷酸化壳聚糖膜可以有效促进牙周组织再生与修复;载入抗菌药物后,效果更明显。Objective To evaluate the effects of posphonized chitosan membrane in guiding tissue regeneration. Methods Three dogs were used to create chronic periodontitis model by surgically placing silk ligaments and high sugar diet, and 24 experimental teeth were randomly assigned to one control group and two treatment groups. The control group was treated by flap operation only（ C group）. One of the treatment groups was cured by guiding tissue regeneration with phosphonized chitosan membrane （ A group）, another was cured by guiding tissue regeneration with drug-loaded phosphonized chitosan membrane（ B group）. The animals were sacrificed at 12 weeks after surgery for histological analysis. Results The amount of new alveolar bone formation, new cementum formation, new connective tissue adhesion in the A group was （ 1.22 ± 0. 15 ） mm, （ 1.00 ± 0. 15 ） mm, （ 1.72 ± 0. 17 ） mm, respectively. The Bgroup：（1.44±0.14）mm,（1.20±0.19）mm,（2.27±0.18）mm;the C group： （0.56±0.16）mm, （0.51 ±0. 14）mm,（ 1.27 ±0. 16） mm. New periodontal tissue formation in the experimental groups were significantly higher than that in the control group. The difference was also significant between the two experimental groups （P 〈 0. 05）. Conclusion Phosphonized chitosan membrane can be used as a biological material for GTR, and the effect of promoting periodontal tissue regeneration may be more obvious after drug-loaded.

关 键 词：引导组织再生 牙周 壳聚糖 

分 类 号：R781.4[医药卫生—口腔医学] R780.2[医药卫生—临床医学]