植物蔗糖转运基因家族功能分化的生物信息学分析  

Bioinformatic Analysis of Functional Divergence for Sucrose Transporter Gene Family in Plants

作　　者：杨泽峰[1] 张丹[1] 敖雁[1] 

机构地区：[1]扬州大学教育部植物功能基因组学重点实验室江苏省作物遗传生理重点实验室,江苏扬州225009

出　　处：《安徽农业科学》2009年第29期14061-14065,共5页Journal of Anhui Agricultural Sciences

基　　金：扬州大学科研创新培育基金资助项目(2008CXJ052)

摘　　要：[目的]用生物信息学方法分析植物蔗糖转运基因家族的功能分化,并明确具有正选择作用的点突变在植物蔗糖转运基因进化过程中的作用。[方法]在基因组水平上对植物的蔗糖转运基因进行鉴别和分类,并利用D IVERGE和PAML软件分别进行功能分化和正选择替代分析。[结果]经基因组检测,发现蔗糖转运基因家族除在单细胞藻类植物中不存在外,在其他植物中均普遍存在;系统发育分析将植物中蔗糖转运基因分成了5个亚群,并发现亚群间存在I型功能分化现象;此外,在3个亚群内检测出了显著的正选择效应。[结论]植物蔗糖转运基因家族在长期进化过程中形成了亚群间的功能分化,同时,具有正选择作用的点突变在该基因家族进化过程中起到重要作用。[ Objective] This study aimed to investigate the funetional divergence of sucrose transporter gene family in plants based on the methods of bioinformaties, and further illustrate the role of point mutations with positive selection to this family during the long period of evolution. [ Method] The members of this family were collected and classified at the genome-wide level, then the tools DIVERGE and PAML were used to test functional divergence and positive selection, respectively. [ Result] The members of suerose transporter gene family were wildly exist in main plant lineages except for unicellular algae. The phylogenetic tree divided the members of this family divided into 5 subgroups, and the statistical evidence of functional divergence between subgroups was found in this analysis. Further more, it was found that 3 subgroups were influeneed by positive selection. [ Conclusion] The sucrose transporters were functional divergent among 5 subgroups, and point mutations with positive selection played important roles during the long evolutionary period of this family.

关 键 词：生物信息学 蔗糖转运体基因家族 功能分化 正选择 

分 类 号：S312[农业科学—作物栽培与耕作技术]