不同抗旱性玉米苗期叶片活性氧代谢对水分胁迫的响应  

Response of Activated Oxygen Metabolism to Water Stress in the Leaves of Different Drought-resistant Corns in Seedling Stage

作　　者：谷岩[1] 梁煊赫[2] 王振民[1] 何文安[1] 赵福林[1] 吴春胜[1] 

机构地区：[1]吉林农业大学作物研究中心,吉林长春130118 [2]吉林省农业科学院,吉林长春130118

出　　处：《安徽农业科学》2009年第29期14089-14091,14117,共4页Journal of Anhui Agricultural Sciences

基　　金：国家粮食丰产工程项目(2004BA520A09)

摘　　要：[目的]抗旱性玉米栽培和育种提供理论依据。[方法]以耐旱品种掖单13和不耐旱品种丹玉13为试材,通过盆栽试验研究了水分胁迫对玉米苗期叶片活性氧代谢水平的影响。[结果]与正常供水处理相比,水分胁迫下掖单13和丹玉13苗期叶片O2产生速率分别升高了28.9%、36.1%(WS 50%)和31.3%、41.9%(WS 35%),叶片H2O2含量分别升高了16.1%、25.4%(WS 50%)和23.7%、29.8%(WS 35%)。中度水分胁迫下掖单13和丹玉13的叶片SOD和POD活性分别比对照提高了22.8%、29.9%和22%、21.7%。与正常供水处理相比,丹玉13和掖单13的叶片MDA含量分别比对照增加了22.7%、14.5%(中度胁迫)和40.2%、15.6%(重度胁迫)。重度水分胁迫下掖单13和丹玉13的AsA和GSH含量分别比对照降低了12.6%、14.2%和33.3%、44.1%。[结论]耐旱性较强的玉米品种在水分胁迫下维持活性氧代谢平衡的能力较强。[ Objective ] The purpose was to supply theoretical basis for the cultivation and breeding of drought-resistant corn. [ Method ] With drought-resistant variety Yedan 13 and drought-susceptible variety Danyu 13 as tested materials, the influences of water stress on the activated oxygen metabolism level in corn leaves in seedling stage were studied through potting experiment. [ Result] Compared with the treatment with normal water supply, under water stress, the production rate of O2 in the leaves of Yedan 13 and Danyu 13 were enhanced by 28.9%, 36.1% （ WS 50% ） and 31.3% , 41.9% （ WS 35% ） resp. and their contents of H2O2 were enhanced by 16.1% , 25.4% （ WS 50% ） and 23.7 % , 29.8% （ WS 35% ） resp. in seedling stage. Under moderate water stress, the activities of SOD and POD in the leaves of Yedan 13 and Danyu 13 were enhanced by 22.8%, 29.9% and 22%, 21.7% resp. in comparison with CK. Compared with the treatment with normal water supply, the contents of MDA in the leaves of Danyu 13 and Yedan 13 were increased by 22.7%, 14.5% （ undei moderate stress） and 40.2%, 15.6% （ under serious stress） resp. in comparison with CK. Under serious water stress, the contents of AsA and GSH in Yedan 13 and Danyu 13 were decreased by 12.6%, 14.2% and 33.3%, 44.1% resp. in comparison with CK. [ Conclusion] The corn variety with stronger drought-resistance had stronger ability on maintaining activated oxygen metabolism balance under water stress.

关 键 词：玉米 抗旱性 水分胁迫 活性氧代谢 

分 类 号：S311[农业科学—作物栽培与耕作技术]