贵州不同海拔地区马铃薯病毒病初步调查及检测鉴定  

Preliminary Investigation and Identification of Potato Virus Diseases at Different Altitudes in Guizhou

作　　者：颜谦[1,2] 黄萍[1,2] 宋吉轩[1,2] 丁映[1,2] 

机构地区：[1]贵州省生物技术研究所,贵州贵阳550006 [2]贵州省马铃薯研究所,贵州贵阳550006

出　　处：《安徽农业科学》2009年第29期14262-14263,共2页Journal of Anhui Agricultural Sciences

基　　金：贵州省科学技术基金"贵州马铃薯易感病毒鉴定及防治技术研究"(黔科合J字[2006]2039号);贵州省科技重大专项:"贵州省马铃薯产业化关键技术研究与示范之子项目1--贵州省马铃薯种质资源库建设"黔科合重大专项字(2008)6009

摘　　要：[目的]调查贵州省不同海拔地区马铃薯病害发生情况,鉴定出贵州省马铃薯易感病毒种类。[方法]采用双抗体夹心酶联免疫吸附测定法(DAS-ELISA)对在黔南州、黔东南州及毕节地区采集的805份马铃薯样品进行病毒检测。[结果]805份马铃薯样品中检出有29份马铃薯X病毒(PVX)为阳性;41份马铃薯Y病毒(PVY)为阳性;285份马铃薯S病毒(PVS)为阳性;23份马铃薯卷叶病毒(PL-RV)为阳性,带病毒率46.95%。[结论]对贵州马铃薯危害较重的病毒种类依次为:马铃薯S病毒(PVS)、马铃薯Y病毒(PVY)、马铃薯X病毒(PVX)、马铃薯卷叶病毒(PLRV)。[ Objective ] The research aimed to investigate the situation of potato virus diseases＇ occurrence and identify the type of the potato susceptible virus at different altitudes in Guizhou Province. [ Method] 805 potato samples collected from Qiarman, Qiandongnan, Bijie Prefecture were detected by DAS-ELISA determination method. [ Result ] Among 805 potato samples, 29 samples were infected by potato virus X （PVX） , 41 samples were infected by potato virus Y （PVY） , 285 samples were infected by potato virus S （PVS） , 23 samples were infected by potato virus （PLRV）. The virus-carrying rate was 46.95%. [ Conclusion] The virus ranking （ from most severe to least） affected potato in Guizhou was as follows： potato virus S（PVS） , potato virus Y （PVY）, potato virus X （PVX）, potato virus （PLRV）.

关 键 词：马铃薯 病毒病 调查 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]