注入不同刺激性液体建立小型猪胃溃疡模型  

Mini-Pig Model of Gastrohelcoma Induced by Different Irritant Reagents

作　　者：田宇瑛[1] 罗明富[1] 徐一慧[1] 张维波[1] 王广军[1] 王瑞红[1] 李翠红[1] 

机构地区：[1]中国中医科学院针灸研究所,北京100700

出　　处：《实验动物科学》2009年第4期32-34,I0002,共4页Laboratory Animal Science

基　　金：科技部973项目资助课题(2006CB504507)

摘　　要：目的摸索并建立小型猪胃溃疡模型。方法使用三种不同刺激性液体通过胃管灌入小型猪胃内,3 h后处死取材,石蜡包埋及冷冻切片,用HE染色,在普通光镜下观察与照相。结果用95%乙醇灌胃后,肉眼观察幽门附近胃黏膜皱襞较完整,色淡红,表面光滑,局部显示有轻微充血、水肿和深红色,镜下显示胃粘膜上皮和胃小凹较完整,但有部分上皮肿胀,出现轻微损伤;用无水乙醇灌胃后,肉眼观察幽门附近胃黏膜皱襞虽较完整,但黏膜暗红颜色的深度和面积均比95%乙醇灌胃模型有明显增加,镜下显示胃黏膜上皮受损,明显变薄,但仍残留少量上皮组织,胃小凹变短,出现明显破损的溃疡面;用36%的冰乙酸灌胃后,肉眼观察胃内壁隆起呈明显白黄色,呈严重缺血坏死状态,镜下显示胃溃疡组织黏膜上皮层损伤严重,部分区域黏膜上皮层完全消失,暴露出固有膜组织。结论用冰乙酸灌胃是比较理想的造模方法。Objective Establishing mini-pig model of gastrohelcoma. Methods Three different irritant reagents were injected into the stomach of mini-pigs through gastric tube and the tissue of stomach was gotten after killing the pig three hours after the injection. The tissue was embedded by paraffin, sliced and dyed by HE which was observed and photo under microscope. Results After injecting 95 % alcohol, the gastric mucous membrane was intact, light red and smooth while only a little congestion, edema and deep red color in loci under macrography. The epidermis and gastric foveola was intact while swelling and slightly damaged in loci under microscope. After injecting anhydrous alcohol, although the gastric mucous membrane was still intact around pylorus, but the membrane with red color was deeper and wider than that of injecting 95 % alcohol. Under microscope, the epidermis was damaged and became obviously thin but still some epidermis was kept. The gastric foveola became short and gastrohelcoma appeared obviously. After injecting 36% glacial acetic acid, the internal wall of stomach hunched with white and yellow color which was ischemia and dead in tissue. The epidermis was damaged severely and disappeared partly while inherent membrane tissue appeared. Conclusion injecting glacial acetic acid through gastric tube is ideal method to make gastrohelcoma.

关 键 词：胃溃疡 小型猪 动物模型 

分 类 号：R-332[医药卫生]