真菌性角膜溃疡的PCR检测  

DETECTION OF FUNGAL CORNEAL ULCER BY POLYMERASE CHAIN REACTION

作　　者：杨建峰 路西林[2] 薛会敏[2] 

机构地区：[1]河北省邯郸市邯钢医院检验科,河北邯郸056001 [2]河北省邯郸市第三医院检验科,河北邯郸056001

出　　处：《河北医科大学学报》2009年第5期473-474,共2页Journal of Hebei Medical University

摘　　要：目的探讨临床拟诊为真菌性角膜溃疡聚合酶链反应(polymerase chain reaction,PCR)快速检测的方法。方法以源于医学条件致病性真菌18s rRNA基因保守区的一对寡核苷酸序列为通用引物,对临床拟诊为真菌性角膜溃疡的38例标本进行PCR检测,并与培养方法进行对比。结果在687 bp处出现DNA扩增带者为阳性。38例临床标本的真菌培养阳性率为60.53%,而经PCR扩增阳性率为81.58%,明显高于真菌培养法(P<0.05)。结论真菌通用引物进行PCR反应检测真菌性角膜溃疡速度快、阳性率高,有助于真菌性角膜溃疡的快速诊断。Objective To find a method for the rapid detection of clinical suspect fungal corneal ulcer by polymerase chain reaction. Methods A pair of oligonucleotide sequences, which was bases on the conserved region of 18s rRNA shared by medically important fungal corneal ulcer,was used as the general primers to amplify the DNAs from clinical suspect fungal corneal ulcer in a PCR assay,and the result was compared with culture. Results A 687 bp specific DNA product was successfully amplified. The positive rate of fungi culture among 38 clinical specimens was 60. 530‰ ,and the positive rate of PCR amplification was 81.58‰. Conclusion PCR with the general primers is fit for rapid detection of fungal corneal ulcer because of quick speed and high positive rate.

关 键 词：聚合酶链反应 真菌 角膜溃疡 

分 类 号：R772.21[医药卫生—眼科]