松嫩草原和黄河入海口罗布麻叶黄酮含量及抗氧化性比较  

Comparisons of contents of total flavonoids from leaves of Apocynum venetum L. collected in Songnen plain and Yellow river mouth area respectively and their antioxidant ability

作　　者：李庆华[1] 魏春雁[2] 黄九林[3] 李建东[3] 

机构地区：[1]吉林医药学院医学实验中心,吉林132013 [2]吉林省农业科学院农业环境与资源研究中心,长春130124 [3]东北师范大学植被生态科学教育部重点实验室,长春130024

出　　处：《食品科技》2009年第10期170-173,共4页Food Science and Technology

基　　金：国家自然科学基金项目(30470180)

摘　　要：超声波辅助提取总黄酮,比色法和HPLC分别测定黄河入海口和松嫩草原野生罗布麻叶总黄酮、槲皮素含量,研究罗布麻叶总黄酮对DPPH·和·OH清除活性。结果表明,松嫩草原和黄河入海口罗布麻叶总黄酮含量分别是5.428～8.071mg/g、12.110mg/g,槲皮素含量分别是2.900～4.531mg/g、5.23mg/g;松嫩草原罗布麻叶总黄酮抗氧化活性较强。Ultrasonic extraction were applied to extract total flavonoids. This study determined the contents of total flavonoids and quercetin from leaves of Apocynum venetum collected in Songnen plain and Yellow river mouth area respectively. Spectrophotometric method has been used to measure total flavonoids, and the content of quercetin determined by HPLC. The antioxidant capacities of the extracts of total flavonoids from leaves of Apocynum venetum towards free radical scavenging were evaluated by using 1,1-diphenyl-2- icrylhydrazyl（DPPH） and hydroxyl radicals. The results showed that both leaves of Apocynum venetum collected in Songnen plain and Yellow river mouth area （2.900-4.531 mg/g and 5.23 mg/g respectively） possessed higher amounts of quercetin and the contents of total flavonoids from theirs were of 5.428-8.071 mg/g and 12.110 mg/g respectively. In addition, leaves of of Apocynum venetum wildly growing in Songnen plain showed higher antioxidant activity as compared to that in Yellow river mouth area.

关 键 词：罗布麻叶 总黄酮 槲皮素 抗氧化活性 

分 类 号：Q946.8[生物学—植物学] TS202.3[轻工技术与工程—食品科学]