气相色谱法快速测定杜仲叶中有机氯菊酯类农药残留  

Rapid Gas Chromatographic Determination of Multi-residue of Organochlorine Pyrethroid Pesticides in Eucommia ulmoides Oliv. leaves

作　　者：王素方 张西安[2] 张东飞[3] 郭俊峰 孙雨安[3] 王国庆[3] 

机构地区：[1]河南出入境检验检疫局检验检疫技术中心,河南郑州450003 [2]开封教育学院自然科学系,河南开封475004 [3]郑州轻工业学院材料与化学工程学院,河南郑州450002

出　　处：《食品科学》2009年第18期323-326,共4页Food Science

基　　金：中国国家认证认可监督管理委员会支持项目(2007B533r);河南省高校科技创新人才支持计划项目(HASTIT)

摘　　要：为了建立ASE-DSPE-GC测定此类农药残留的方法。将加速溶剂萃取(accelerated solvent extraction,ASE)与分散固相萃取(dispersive solid phase extraction,DSPE)结合,用于杜仲叶中有机氯菊酯类农药残留的同时提取净化,用气相色谱法(gaschromatography,GC)检测。采用该法对杜仲叶中14种有机氯菊酯类农药进行测定,目标物平均回收率为76.8%～118.9%,相对标准偏差(RSD)为2.6%～13.9%,定量检出限为10ng/g,线性范围为2～100ng/ml,标准工作曲线线性相关系数R≥0.998。结果表明,ASE-DSPE样品前处理效率高,该法可用于同时测定杜仲叶中的有机氯菊酯类农药残留的提取和净化。A rapid multi-residue method based on accelerated solvent extraction （ASE） combined with dispersive solid-phase extraction （DSPE） followed by gas chromatography with electron-capture detection （GC-ECD） was developed for the determination of 14 organochlorine pyrethroid pesticides in Eucommia ulmoides Oliv. leaves. The average fortified recoveries of the pesticides at three levels of 10, 20 and 50 ng ranged from 76.8% to 118.9%, with a RSD between 2.6% and 13.9%. The quantitative detection limit of this method was 10 ng/g. In the concentration range of 2-100 ng/ml, most of the pesticides displayed a good linearity with peak area, with a correlation coefficient （R） higher than 0.998. The combination of ASE and DSPE gives a highly effective pretreatment for the rapid GC-ECD detection of multi-residue of organochlorine pyrethroid pesticides in Eucommia ulmoides Oliv. leaves.

关 键 词：加速溶剂萃取(ASE) 分散固相萃取(DSPE) 气相色谱法(GC) 有机氯菊酯 

分 类 号：O657.7[理学—分析化学] S481.8[理学—化学]