离子色谱法与硫酸钡比浊法测定琼胶糖中硫酸基含量的比较研究  

Comparison of Ion Chromatographic and Barium Sulfate Turbidimetric Determination of Sulfate Group in Agarose

作　　者：杨贤庆[1] 赵永强[1,2] 戚勃[1] 李来好[1] 陈胜军[1] 岑剑伟[1] 

机构地区：[1]中国水产科学研究院南海水产研究所,广东广州510300 [2]广东海洋大学食品科技学院,广东湛江524025

出　　处：《食品科学》2009年第18期340-343,共4页Food Science

基　　金：国家"863"计划项目(2007AA10Z345);广东省科技计划项目(2007A032600003);广东省海洋渔业科技推广专项项目(A200899E01);中国水产科学研究院南海水产研究所中央级公益性科研院所专项资金项目(2007ZD06)

摘　　要：以琼胶糖为原料,样品用干法灰化法处理后分别以超纯水和1.0mol/L盐酸溶解定容,利用离子色谱法和硫酸钡比浊法测定其硫酸基含量(以SO42-计)。结果表明,离子色谱法SO42-在1.0～15.0mg/L范围内线性关系良好(R2=0.9996),硫酸钡比浊法SO42-在6.0～30.0mg/L范围内线性关系良好(R2=0.9991);对同种琼胶糖样品进行6次重复测定,离子色谱法相对标准偏差为1.40%(n=6),具有较高的精密度,硫酸钡比浊法相对标准偏差为3.51%(n=6);对两种不同的琼胶糖样品分别进行两个水平的加标回收率实验,离子色谱法回收率为86.33%～97.40%,硫酸钡比浊法回收率为84.17%～96.67%。因此,离子色谱法和硫酸钡比浊法均能准确测定样品中硫酸基含量。Agarose was dry ashed and the ash was separately diluted with ultrapure water and 1.0 mol/L hydrochloric acid to volume for measuring sulfate group content by ion chromatography （IC） and barium sulfate turbidimetry （BST）. The IC method exhibited a good linear relationship in the SO4^2- concentration range of 1.0-15.0 mg/L, with a correlation coefficient of 0.9996, the RSD of 6 repetitions was 1.40%, indicating a high precision, and the recoveries in at two spiked levels were from 86.33% to 97.40%; As for the BST method, the linear range was 6.0-30.0 mg/L and the correlation coefficient was 0.9991, the RSD of 6 repetitions was 3.51%, and the recoveries in at two spiked levels were from 84.17% to 96.67%.These results show that both IC and BST can provide an accurate determination of sulfate group in agarose.

关 键 词：琼胶糖 硫酸基 离子色谱法 硫酸钡比浊法 

分 类 号：O657.75[理学—分析化学] O657.32[理学—化学]