吸烟与温石棉单独及联合作用对人胚肺细胞DNA链断裂的影响  

The separate or combined effect of cigarette smoking and chrysotile on DNA strand breaks in human embryo lung cells

作　　者：王起恩 樊晶光 张侠 刘世杰 

出　　处：《中华劳动卫生职业病杂志》1998年第5期273-276,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的研究吸烟与温石棉对细胞的遗传毒性。方法采用单细胞凝胶电泳技术（ＳＣＧＥ），在体外实验中测定了香烟烟雾溶液（ＣＳＳ）与温石棉（ＣＨ）单独及联合作用于人胚肺（ＨＥＬ）细胞时ＤＮＡ链断裂的情况。结果ＣＳＳ与ＣＨ单独与ＨＥＬ细胞作用１小时，均可使ＤＮＡ链断裂明显增多，当二者以３种不同的剂量组合联合作用时，均可协同增加ＤＮＡ链断裂。结论ＣＳＳ和ＣＨ单独作用均为肿瘤启动剂；当二者联合作用时可协同启动肿瘤的发生。Objective To study the genetic toxic effect of cigarette smoking and chrysotile on human embryo lung(HEL) cells.Methods DNA strand breaks in HEL cells were determined by single cell gel electrophoresis(SCGE) in vitro when HEL cells were exposed to cigarette smoking solution(CSS) and chrysotile(CH) separately or simultaneously.Results DNA strand breaks significantly increased when HEL cells were exposed to CSS or CH separately,and synergistically increased when exposed to CSS and CH simultaneously.Conclusion Both CSS and CH may act as initiator in carcinogenesis,and may synergistically initiate tumors when CSS and CH were exposed simultaneously.

关 键 词：吸烟 温石棉 DNA损伤 肺癌 致癌性 联合作用 

分 类 号：R734.202[医药卫生—肿瘤] R730.231.1[医药卫生—临床医学]