注射用川芎提取物提取纯化方法的优选  

Optimization of extraction and purification of Rhizoma Chuanxiong extract for injection

作　　者：初阳[1,2] 宋洪涛[1] 李丹[3] 鄢璐[3] 刘丹[3] 陈大为[3] 

机构地区：[1]南京军区福州总医院药学科,福建福州350025 [2]中国医科大学附属第一医院药剂科,辽宁沈阳110001 [3]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《中草药》2009年第10期1573-1576,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30200363)

摘　　要：目的优选注射用川芎提取物的提取纯化方法。方法以阿魏酸提取率为考察指标,优选提取工艺;以阿魏酸的转移率、杂质的清除效果和提取物的复溶性作为考察指标,应用大孔吸附树脂和聚酰胺吸附法对川芎提取物进行纯化,优选纯化条件。结果以70%乙醇回流提取2次,阿魏酸提取率较高;将大孔吸附树脂法和聚酰胺吸附法联合使用纯化川芎提取物,阿魏酸的转移率可达90.3%,杂质清除效果和提取物复溶性良好。结论以70%乙醇进行提取,大孔吸附树脂和聚酰胺吸附法联用进行纯化,所得川芎提取物可达到注射用要求。Objective To develop the extraction and purification method for the active fraction of Rhizoma Chuanxiong extract for injection. Methods The extracting technology was optimized by using the extraction rate of ferulic acid as the estimated targets. The conditions of purification were confirmed through applying the macroporous resin and polyamide with using the transfering rate of ferulic acid, scavenging effect of impurities and reconstitution of extract as the estimated indexes. Results The extraction rate of ferulic acid from Rhizoma Chuanxiong was higher with 70% alcohol as extracting solvent for twice. After the extract of Rhizoma Chuanxiong being purified with macroporous resin and polyamide adsorption, the transfering rate of ferulic acid was up to 90.3~, the scavenging effect of both impurities and re- constitution of extract were good. Conclusion The extract of Rhizoma Chuanxiong could meet the injection standard by using 70% alcohol as extracting solvent, then applying macroporous resin and adsorption of polyamide as purification methods.

关 键 词：注射用川芎提取物 提取 纯化 阿魏酸 

分 类 号：R284.2[医药卫生—中药学] R286.02[医药卫生—中医学]