失血性休克大鼠血清对人脐静脉内皮细胞内皮细胞蛋白C受体表达的影响  

Effects of the Serum in Hemorrhagic Shock Rats on Endothelial Cell Protein C Receptor of Human Umbilical Vein Endothelial Cells

作　　者：张近波[1] 潘景业[1] 张小乐 李智强[1] 陈洁[1] 沈晔[1] 吴双华[1] 

机构地区：[1]温州医学院附属第一医院ICU室,浙江温州325000 [2]滦南县医院血液科,河北滦南063500

出　　处：《中华中医药学刊》2009年第10期2111-2114,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省自然科学基金项目(Y207635)

摘　　要：目的:探讨失血性休克大鼠血清对人脐静脉内皮细胞内皮细胞蛋白C受体(EPCR)表达的影响。方法:采用酶联免疫吸附试验(ELISA)、逆转录聚合酶链反应(RT-PCR)、WESTERN BLOT技术分别对正常对照组、失血性休克(3h、12h、24h、72h)组大鼠血清培养的人脐静脉内皮细胞(HUVEC)可溶性EPCR(sEPCR)水平和EPCR mRNA及蛋白表达进行检测。结果:与正常对照组比较,休克3h、72h组sEPCR、EPCR的mRNA、蛋白含量均无明显变化(P均>0.05),休克12h、24h组sEPCR、EPCR的mRNA含量均显著增高(P均<0.05),EPCR的蛋白含量均显著降低(P均<0.05)。结论:失血性休克大鼠血清可以从基因、蛋白水平影响人脐静脉内皮细胞EPCR的表达。Objective：To investigate the effects of the serum in hemorrhagic shock rats and Shenfu injection on endothelial cell＇s thrombomodul and endothelial cell protein C receptor. Methods：The soluble endothelial protein C receptor （sEPCR） in supernatant , EPCR mRNA and protein of human umbilical vein endothelial cell were detected by enzyme linked Immunosorbent assay （ELISA）, reverse transcription polymerase chain reaction （RT- PCR） , western bloting （ WB ） in endothelial cell culturing with serum, normal control, hemorrhagic shock （3h, 12h, 24h ,72h） group respectively. Results ： The mean levels of sEPCR, the expression of EPCR mRNA and protein were no significant differences in hemorrhagic shock（3h,72h） group than that in normal control group （ all P〉0.05）. The mean levels of sEPCR and the expression of EPCR mRNA were significantly higher in hemorrhagic shock（12h,24h） group than that in normal control group （ all P 〈 0.05 ）, while the level of protein was lower than that in normal control group （ both P 〈 0.05 ）. Conclusion： These data suggest that serum in hemorrhagic shock rats could effect sEPCR ,EPCR mRNA and protein of human umbilical vein endothelial cells expression.

关 键 词：失血性休克 内皮细胞蛋白C受体 

分 类 号：R-33[医药卫生]