亚慢性暴露高频电磁场对雌性大鼠性腺的毒性作用  

Female genital toxicities of high-frequency electromagnetic field on rats

作　　者：徐幽琼[1] 郑能雄[1] 李宝华 张文昌[3] 林新国[1] 汪家梨[3] 陈国兴[1] 

机构地区：[1]福建省福州市疾病预防控制中心,350004 [2]福州市马尾区疾病预防控制中心 [3]福建医科大学公共卫生学院职业与环境卫生学系

出　　处：《中华劳动卫生职业病杂志》2009年第9期544-548,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：福建省福州市科技局重点项目资助项目（2005.17）

摘　　要：目的研究亚慢性暴露高频电磁场（HF—EMF，30MHz）对雌性大鼠性腺的毒性作用。方法选用清洁级Wistar雌性大鼠60只，按体重随机分为5组，暴露剂量分别为0、25、100、400、1600V/m，大鼠全身暴露于HF—EMF，8h／d，每周连续5d，停2d，共暴露56d。辐射期间大鼠每3天称1次体重，第48天开始观察大鼠动情周期的变化，56d后待大鼠处于动情期时取血清和卵巢，采用放射免疫法测定相关激素，HE染色法计算卵巢各级卯泡的数目构成比，用电子显微镜观察细胞超微结构。结果各剂量组大鼠体重与卵巢湿重及脏器系数的差异无统计学意义（P〉0．05）；100、400、1600 V／m组大鼠动情前期时间分别为（15．00±5．06）、（11．40±2．05）、（10．56±0．96）h，明显短于对照组[（18．70±2．96）h]，差异有统计学意义（P〈0．01）；400、1600V／m组大鼠动情间期时间分别为（101．20±17．81）、（115．33±19．28）h，明显长于对照组[（69．80±11．42）h]，差异有统计学意义（P〈0．01）。400、1600V／m组的黄体生成素（LH）水平[分别为（11．02±1．11）、（14，70±1．94）mU／ml]明显高于对照组[（8．70±0．53）mU／ml]，差异有统计学意义（P〈0．01）；而雌激素（E2）水平[分别为（57．16±31．56）、（50．57±25．16）pg／ml]明显低于对照组[（95．04±32．62）pg／ml]，差异有统计学意义（P〈0．01）；400、1600V／m组黄体数目构成比（分别为19．75％、19．04％）明湿高于对照组（14．01％），差异有统计学意义（P〈0．01），100、400、1600V／m组闭锁卵泡数目构成比分别为8．45％、9．95％、IL70％，明显高于对照组（7．72％），差异有统计学意义（P〈0．01）；而400、1600V/m组成熟卯泡数目构成比（分别为1．50％、1．55％）明屈低于对照组（3．36％），差异有统计学意义（P〈0．01）；400、1600 V／m组初／�Objective To study on the effects of sub-chronic exposures to high-frequency electromagnetic field （HF-EMF） on the estrous cycle, ovarian pathological changes and related hormones and preliminarily investigate the female genital toxicities of HF-EMF in tats. Methods 60 Wistar female adult rats were randomly divided into five groups based on hody weight and radiated with different levels of 30 MHz electromagnetic field （0, 25, 100, 400 and 1600 V/m） for eight hours daily. Weekly the rats were continuously exposed five days. From the 48th day the four stage of estrus cycle were observed with smear method of the vaginal cell. Fifty-six days later the serum levels of sexual hormones were detected with the radioimmnnoassay on estrus stage. The constituent ratio of the distinct follicle number on ovaries were observed with the HE staining and the uhramicrostructure was ohservcd with the transmission electron microscope. Meanwhile, the ovarial humid weight and organ coefficient were observed. Results There was no significant difference in ovarial humid weight and organ coefficient between the exposure and the control groups. The time of preoestrus in the 100 V/m group, the 400 V/m group and the 1600 V/m group was decreased significantly [（15.00±5.06）,（11.40±2.05 ） and （10.56±0.96） h in the exposure group compared with （18.70±2.96） h in the control group, P〈0.01], and the time of Anestrum in the 400 V/m group and the 1600 V/m group were increased significantly [（ 101.20±17.81 ） and （115.33±19.28） h in the exposure group compared with （69.80±11.42） h in the control group, P〈0.01 ）]. Serum LH in the 400 V/m and 1600 V/m group was increased significantly[（ 11.02±1.11 ） and （14.70±1.94） mU/ml in the exposure groups compared with （8.70±0.53） mU/ml in the control group, P〈0.01], and serum E2 was decreased significantly [（57.16±31.56） and （50.57±25.16） pg/ml in the exposure groups compared with （95.04±32.62） pg/ml in the control group, P〈0.01 ].

关 键 词：电磁场 动情期 性激素类 卯泡闭锁 

分 类 号：R686[医药卫生—骨科学]