炎症性肠病患者外周血单个核细胞和血浆中肿瘤坏死因子受体相关因子-6的激活  

Activation of TRAF-6 in Peripheral Blood Mononuclear Cells and Plasma in Patients with Inflammatory Bowel Disease

作　　者：沈骏[1,2] 冉志华[1,2] 童锦禄[1,2] 聂芳[1,2] 蔡青[1,2] 黄美兰[1,2] 王天蓉[1,2] 钱孝先[1,2] 张凤[1,2] 

机构地区：[1]上海交通大学医学院附属仁济医院消化内科 [2]上海市消化疾病研究所,上海200001

出　　处：《临床消化病杂志》2009年第5期259-262,共4页Chinese Journal of Clinical Gastroenterology

基　　金：上海市科委重点实验室项目(06DZ22027)

摘　　要：目的探讨肿瘤坏死因子受体相关因子-6(tumor necrosis factor receptor-associated factor-6,TRAF-6)在炎症性肠病患者外周血单个核细胞和血浆的表达,及其与内镜下疾病活动性的相关性。方法应用酶联免疫吸附试验(ELISA)分析炎症性肠病患者和正常对照者血浆中TRAF-6蛋白的表达,SYBR-green real time PCR方法分析炎症性肠病患者和正常对照者外周血单个核细胞中TRAF-6mRNA的表达。数据处理使用Graph Pad Prism5。结果克罗恩病患者(P=0.000)和溃疡性结肠炎患者(P=0.000)血浆TRAF-6水平显著高于正常对照者,但是克罗恩病患者(r=-0.114,P=0.377)以及溃疡性结肠炎患者(r=0.044,P=0.727)血浆TRAF-6表达水平和内镜下疾病活动指数均无显著的相关性。克罗恩病患者(P=0.000)和溃疡性结肠炎(P=0.000)患者外周血单个核细胞TRAF-6mRNA表达均显著高于正常对照者外周血单个核细胞中mRNA的表达。结论炎症性肠病患者血浆和外周血单个核细胞中均存在TRAF-6的激活,但血浆TRAF-6的高表达不能反映内镜下的疾病活动程度。Objective To investigate the expression of tumor necrosis factor receptor-associated factor- 6 （ TRAF- 6 ） in peripheral blood mononuclear cells and plasma in patients with inflammatory bowel disease, find the difference between the expression of TRAF6 in patients with inflammatory bowel disease and in healthy controls, and analyze the correlation between plasma TRAF-6 levels and endoscopic disease activity. Methods Enzyme-linked immuno sorbent assay （ELISA） was applied to evaluate plasma TRAF-6 levels in patients with inflammatory bowel disease and in healthy controls. SYBR-green real time PCR was applied to access TRAF-6 mRNA expression in peripheral blood mononuclear cells in patients with inflammatory bowel disease and healthy controls. Data were analyzed by using Graph Pad Prism 5. Results Plasma levels of TRAF-6 were significantly higher in Crohn＇ s disease （ P = 0. 000） and ulcerative colitis （ P = 0. 000 ） than in healthy controls. However, no significant correlation was indicated between plasma TRAF-6 and endoscopic disease activity in patients with Crohn＇s disease（ r = - 0.114, P = 0. 377 ） or in patients with ulcerative colitis （ r = 0. 044, P = 0.727 ）. Furthermore, TRAF-6 mRNA expression in peripheral blood mononuclear ceils was significantly higher in patients with Crohn＇ s disease and ulcerative colitis than in healthy controls （ all P = 0. 000）. Conclusion TRAF-6 activated in plasma and peripheral blood mononuclear cells in patients with inflammatory bowel disease. However, plasma TRAF-6 levels can not reflect endoscopic disease activity.

关 键 词：肿瘤坏死因子受体相关因子-6 克罗恩病 溃疡性结肠炎 内镜下疾病活动程度 

分 类 号：R574[医药卫生—消化系统]