精子蛋白17异常表达与卵巢癌细胞对铂类药物耐药的相关性研究  

Relationship of overexpression of sperm protein 17 with chemoresistance of ovarian cancer cells to platinum

作　　者：李芳秋[1] 韩艳玲[1] 刘群[1] 周万青[1] 

机构地区：[1]南京军区南京总医院全军临床检验医学研究所,江苏南京210002

出　　处：《中华肿瘤防治杂志》2009年第17期1281-1283,共3页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(30670599);南京军区医学科技"十一五"研究计划重点项目(06Z43);国家973计划项目(2006CB705707)

摘　　要：目的:观察精子蛋白17(Sp17)的异常表达对体外培养的卵巢癌细胞顺铂和卡铂耐药性的影响。方法:将Sp17基因转染卵巢癌细胞系HO8910,采用流式细胞术测定目的蛋白的表达,获得稳定高表达Sp17的卵巢癌细胞系HO8910/Sp17。MTT法检测铂类药物对转染细胞HO8910/Sp17的生长抑制作用并与未转染的亲代细胞进行比较。结果:在有效剂量范围内,顺铂和卡铂对HO8910/Sp17细胞的抑制率明显低于亲本细胞HO8910,P<0.05。耐药指数顺铂1.4,卡铂1.38。结论:Sp17明显增加卵巢癌细胞对铂类化疗药物的耐药性。OBJECTIVE： To observe the effect of overexpres sion of human sperm protein 17（Sp17） on the chemoresistance of ovarian cancer ceils to cisplatin and earboplatin. METHODS： hSpl7 cDNA was transfected into human ovarian carcinoma cell line HO8910 to establish a cancer cell line with stable overexpression of Sp17. The expression of interest protein was examined by flow cy tornetry. The inhibitory effects of carboplatin and cisplatin on trans feted cancer cells HO8910/Sp17 and parent HO8910 were detected by the colorimetric MTT assay. RESULTS： The inhibition ratios of carboplatin and cisplatin on HO8910/Sp17 cells were significantly lower than those on parent cells （P〈0.05） at appropriate concentrations. The resistance indexes of cisplatin and carboplatin were 1.4 and 1.38, respectively. CONCLUSION： The overexpression of Sp17 can increase the ehemoresistance of ovarian cancer cells to platinum.

关 键 词：卵巢肿瘤/药物疗法 载体蛋白质类/生物合成 顺铂/药理学 卡铂/药理学 

分 类 号：R737.31[医药卫生—肿瘤]