吉西他滨白蛋白纳米粒对胰腺癌微血管生成的影响  

Effect of gemcitabine-loaded albumin nanoparticles on the micro-angiogenesis of pancreatic cancer

作　　者：李金明[1,2] 何航[1] 刘辰[1] 龙江[1] 金忱[1] 傅德良[1] 倪泉兴[1] 

机构地区：[1]复旦大学附属华山医院普外科复旦大学胰腺病研究所,上海200040 [2]上海交通大学医学院附属新华医院肛肠外科

出　　处：《外科理论与实践》2009年第5期523-526,共4页Journal of Surgery Concepts & Practice

基　　金：上海市经委重点产业技术产学研联合攻关项目(沪产学研06-23;07ZH028);上海市科委重大科技攻关项目(08431902500)

摘　　要：目的:研究吉西他滨白蛋白纳米粒(GEM-NP)对胰腺癌微血管生成的影响。方法:以人胰腺癌裸鼠移植瘤为对象,分为5个给药组:110 nm-GEM-NP组(n=6)、406 nm-GEM-NP组(n=6)、GEM组(n=6)、NP组(n=6)及无药对照组(n=6);运用CD34免疫组化检测微血管密度(MVD),RT-PCR法检测血管内皮生长因子(VEGF)mRNA的表达。结果:5个组给药后MVD依次为1.80±0.90、2.58±1.10、3.92±1.13、6.61±1.65及9.56±2.99;其中3个GEM阳性给药组与2个无GEM组间均有显著差异(P<0.05),110 nm-GEM-NP组与GEM组间也具有显著差异(P<0.05)。而各组间VEGF mRNA表达差异无统计学意义(P>0.05)。结论:GEM-NP对胰腺癌微血管生成有较显著的抑制效应。Objective To investigate the effect of gemcitabine-loaded albumin nanoparticles （GEM-NP） on the micro-angiogenesis of pancreatic cancer. Methods Nude mice bearing transplanted tumor of human pancreatic cancer （PANC-1 xenografts） were divided into 5 groups： 110nm-GEM-NP group（n=6）, 406nm-GEM-NP group（n=6）, GEM group （n=6）, NP group（n=6） and control group（n=6）, which were separately treated with GEM-NP of 110 nm diameter, GEM-NP of 406 nm diameter, pure GEM, albumin-free nanoparticles and normal saline. The microvessel density （MVD） of the tumors in each group were assessed by immunohistochemistry method with CD34 antibody, and the expression of VEGF mRNA were detected by RT-PCR. Results MVD of the 5 groups were 1.80±0.90,2.58±1.10,3.92±1.13,6.61±1.65 and 9.56±2.99 respectively, with significant difference between the groups treated with GEM and the groups without GEM（P〈 0.05）, and there was also a significant difference of MVD between the 110nm-GEM-NP group and the GEM group （P〈 0.05）. However, there was no significant difference of the VEGF mRNA expression among these groups （P〈0.05）. Conclusions GEM-NP have stronger depressive effect on the micro-angiogenesis of pancreatic cancer.

关 键 词：吉西他滨 纳米粒 白蛋白 胰腺肿瘤 微血管 

分 类 号：R735.9[医药卫生—肿瘤]