聚焦超声对S180肿瘤细胞膜理化性质的影响  

The Effect of Focused Ultrasound on the Physicochemical Properties of Sarcoma 180 Cell Membrane

作　　者：李焘[1,2] 郝巧[2] 王筱冰[2] 刘全宏[2] 

机构地区：[1]陕西师范大学药用植物资源与天然药物化学教育部重点实验室,西安710062 [2]陕西师范大学生命科学学院,西安710062

出　　处：《生物医学工程学杂志》2009年第5期941-946,共6页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30270383)

摘　　要：初步探讨频率为2.2MHz的聚焦超声对S180肿瘤细胞膜的损伤作用及其可能的作用机制。台盼兰拒染法检测不同处理后细胞的相对存活率;流式细胞仪和荧光显微镜观察辐照后胞内异硫氰酸荧光素钠标记的右旋糖酐的量以检测膜通透性的变化;检测不同处理后细胞悬液中乳酸脱氢酶的释放量以测定膜完整性的改变;硫代巴比妥酸法检测MDA含量以反映膜脂质过氧化的程度。超声导致细胞膜通透性、完整性及流动性均发生改变,其损伤程度与超声有一定的时效关系,其具体作用机制可能与细胞膜脂质过氧化有关。This study was amied to detect the changes in the cell membrane of Sarcoma 180 （S180） cells induced by focused ultrasound and to probe the underlying mechanism. The viability of tumor cells was examined at various intensities and different treatment times by ultrasound at the frequency of 2.2MHz. Flow cytometry and fluorescence microscopy were used to detect the loading of fluorescein isothiocyanate dextran （FD500） which signifies the change of membrane permeability. The results showed that after the cells were treated by ultrasound, especially when irradiated for 60s, the number of fluorescent cell, which represented the transient change of membrane permeabilization with cell survival, increased significantly. Then the damage of cell membrane was evaluated by the measurement of lactate dehydrogenase （LDH） release which became more severe as the radiation time was increasing. The generation of lipid peroxidation was estimated using the Thibabituric Acid （TBA） method after irradiation. The results reveal that the instant cell damage effects induced by ultrasound may be related to the improved membrane lipid peroxidation levels post-treatment. The physicochemieal properties of S180 cell membrane were changed by focused ultrasound. The findings also imply an exposure time-dependent pattern and suggest that the lipid peroxidation produced by acoustic cavitation may play important roles in these actions.

关 键 词：聚焦超声 S180肿瘤细胞 细胞膜 

分 类 号：R730.2[医药卫生—肿瘤]