能量可控陡脉冲抑制乳腺癌细胞增殖活性的机制初探  

The Proliferating Ability Inhibited by Energy Controllable Steep Pulse in Breast Cancer Cell Line and Its Mechanism

作　　者：朱赟珊[1] 胡丽娜[2] 董晓静[3] 罗小东[3] 孙才新[4] 

机构地区：[1]浙江大学医学院附属第二医院妇科,杭州310009 [2]四川大学华西第二医院妇产科,成都610041 [3]重庆医科大学附属第二医院妇产科,重庆400010 [4]重庆大学,重庆400044

出　　处：《生物医学工程学杂志》2009年第5期1060-1063,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30371619)

摘　　要：观察能量可控陡脉冲对乳腺癌细胞增殖抑制作用以及相关基因表达的影响,探讨能量可控陡脉冲抑制肿瘤细胞增殖的可能机制。能量可控陡脉冲作用于人乳腺癌细胞系MDA-MB-231,于作用后24h应用TUNEL法观察肿瘤细胞凋亡,RT-RCR方法测定处理组和对照组中Rb基因及转录调节因子E2F1基因表达的改变。陡脉冲对乳腺癌细胞的增殖有明显抑制作用,与对照组相比处理组细胞数目明显减少,TUNEL阳性细胞显著增多,处理组Rb基因mRNA表达明显升高、E2F1mRNA表达明显下降。能量可控陡脉冲具有抑制肿瘤细胞增殖,诱导肿瘤细胞凋亡的作用,Rb基因、EF基因可能参与了能量可控陡脉冲抑制恶性肿瘤细胞增殖的过程。This was a study aimed to observe the proliferating ability inhibited by energy controllable steep pulse （ECSP） and to detect the expression of gene with relation to the proliferating ability of the tumor in breast cancer cell line; the possible mechanisms were also addressed. Human breast cancer cell line MDA-MB-231 was treated with ECSP; the apoptosis and the expression of tumor suppressor gene - Rb genes and Ez F1 genes in ECSP group and control group were detected by TUNEL staining and Reverse Transcripitional PCR respectively. ECSP was found to inhibit the proliferating ability of breast cancer cells markedly; the cell amount in ECSP group decreased and the TUNEL positive cells increased obviously, compared to control; 24 hours after treatment the expression of Rb genes mRNA increased, whereas the expression of E2 F1 mRNA decreased. These findings indicate that the proliferating ability of breast cancer cells can be inhibited by ECSP markedly, the apoptosis of breast cancer cell can be induced by ECSP, and the Rb genes and E2F1 genes may be involved in the course.

关 键 词：能量可控陡脉冲 乳腺癌 增殖 

分 类 号：R737.9[医药卫生—肿瘤]