肾缺血再灌注对大鼠室旁核电活动的影响  

Effects of renal ischemia/reperfusion on the electrical activity of paraventricular nucleus in rats

作　　者：刘希秀[1] 冉兵[1] 

机构地区：[1]泸州医学院基础医学院生理学教研室,泸州646000

出　　处：《神经解剖学杂志》2009年第5期537-540,共4页Chinese Journal of Neuroanatomy

摘　　要：为了观察肾缺血-再灌注对室旁核电活动的影响,探讨中枢神经活动在急性缺血再灌注肾损伤发生、发展中的作用和机制。本实验通过夹闭大鼠一侧肾动脉30min、再灌注30min的肾缺血再灌注模型,同步动态记录肾动脉夹闭致缺血-再灌注性肾损伤大鼠室旁核的放电频率、放电周期、放电积分等参数的变化。结果显示:(1)与对照组相比,肾缺血瞬间室旁核电活动受到抑制,缺血5min后电活动逐渐增强并维持在较高水平;(2)去夹闭再灌流瞬间放电立即减少,再灌注5min后放电又逐渐增强,并于再灌注20min～25min时室旁核放电达最强,与缺血前及缺血中各个观测点相比,均有显著性差异(P<0.05～0.01)。上述结果提示肾脏在缺血和缺血-再灌注过程中可引起室旁核电活动发生周期性变化,其中再灌注后对室旁核电活动的影响较缺血的影响更为明显。To observe the effects of renal ischemia/reperfusion （I/R） injury on the electrical activities of paraventricular nucleus （ PVN）, and probe into the role and its mechanism of central nerve activity in renal isehemia reperfusion rats. In this experiment, rat I/R model was made by occluding single lateral renal artery for 30 minutes, then reperfusion for 30 minutes, and synchronous dynamically recorded the discharge frequency, cycle and integral of PVN. The results showed that： （ 1 ） the moment the renal artery was occluded, the electrical activity of PVN was inhibited compared with that of control groups, whereas 5 minutes later, it enhanced gradually and maintained at the high level; （2） The relief of occluding resulted in immediate decrease of discharge, and the discharge enhanced again five minutes later. The discharges of PVN reached the peaks at 20-25 minutes after reperfusion. The electrical activities of reperfusion period has statistical significance difference （ P 〈0.05-0.01 ） compared with those of all time points before and during ischemia. The above results indicate that during renal ischemia and ischemia/reperfusion, the discharges of PVN show cycle variation, especially the ischemia/repeffusion impact greater than ischemia on discharges of PVN.

关 键 词：肾脏 缺血 缺血-再灌注 室旁核 

分 类 号：R363[医药卫生—病理学]