茶树体内铝形态及铝累积特性  

Forms and Accumulation of Aluminum in Tea Plant (Camellia sinensis)

作　　者：孙婷[1] 刘鹏[1] 郑人卫[1,2] 谢忠雷[3] 罗虹[1] 

机构地区：[1]浙江师范大学植物学实验室,浙江金华321004 [2]浙江师范大学化学系,浙江金华321004 [3]吉林大学环境与资源学院,吉林长春130026

出　　处：《作物学报》2009年第10期1909-1915,共7页Acta Agronomica Sinica

基　　金：国家自然科学基金资助项目(30540056;40573052);浙江省自然科学基金资助项目(303461;504135)资助

摘　　要：设置不同Al3+浓度对青茶进行50d处理,调查茶树铝含量和铝的化学配位形态。结果表明,茶树体内的铝大多以有机态或螯合态形式存在;茶树老叶具有高积累铝的特性,但以5mmolL-1铝处理时,运输到叶片的铝减少,积累于茶树根部的铝增多。利用27AlNMR技术检测表明,茶树各器官中普遍存在Al13的强烈共振吸收峰;在各器官中还出现–0.38×10-6处和–0.17×10-6处的微弱吸收峰,为目前尚未检出的铝络合物形式;在5mmolL-1Al3+处理下,青茶老叶中含有更多的Al-复合物,包括Al-草酸盐(1∶2)、Al-草酸盐(1∶2)和Al-磷酸复合物,说明茶树体中的铝通过与其他物质形成络合物以降低铝的毒性。Aluminum toxicity is a major limiting factor affecting yield and quality of crops in acid soil. Camellia sinensis is an Al-accumulating plant grown healthily even in strong acid soil with high aluminum content. The study of the forms and accumulation of aluminum will be helpful to reveal the Al-toleranting mechanism of tea plant. In this paper, the content of Al^3＋ in tea plants was determined after Camellia sinensis wastreated for 50 d with different concentrations of Al^3＋ in culture solution. Besides, the forms of Al were analyzed in the roots, stems and leaves of tea plants by the nondestructive ^27Al NMR with high accuracy. The old leaves of tea plant were the major Al-accumulating organ, whereas, roots would take the position of it and accumulate more aluminum when the plant was treated with 2 mmol L^-1 Al^3＋. ^27Al NMR test showed that the dissoluble aluminum in tea root, stem and leaf existed mainly in the form of [AlO4Al12（OH）24（H2O）12]^7＋（Al13）, whose chemical shift was 63×10^-6, and it was the first discovery in tea plant. Besides of Al13, there were two weak absorption peaks of -0.38×10^-6 and -0.17×10^-6 in three organs tested, which wereundetected aluminum complexes. Other three complexes appeared in old leaf when treated by 5 mmol L^-1 Al^3＋, which were Al-oxalate （1：1）, Al-oxalate （1：2）, and Al-phosphate, and the chemical shifts were respectively 6.4×10^-6, 12.7×10^-6 and -6.2×10^-6. In summary, most of Al^3＋in tea plant exist in chelate complexes or organic matter, which can be considered as one of Al-accumulating and Al-tolerance mechanism of tea plant.

关 键 词：27AlNMR 茶树 铝形态 铝累积 

分 类 号：S571.1[农业科学—茶叶生产加工] X132[农业科学—作物学]