电针足三里穴对大鼠40％血容量失血口服补液时胃排空和胃血流量的影响  

Effects of electro-acupuncture at Zusanli point (足三里穴) on gastric emptying and gastric blood flow in oral fluid resuscitation of 40% blood volume loss in rats

作　　者：张立俭[1] 石现[1] 周国勇[2] 侯经元[2] 包呈梅[2] 胡森[2] 

机构地区：[1]解放军总医院针灸科,北京100853 [2]解放军总医院第一附属医院烧伤研究所休克与多器官障碍实验室,北京100048

出　　处：《感染．炎症．修复》2009年第3期143-145,共3页Infection Inflammation Repair

基　　金：全军医学科研"十一五"专项课题(06Z055)

摘　　要：目的:研究电针足三里穴对40%血容量失血大鼠早期口服葡萄糖-电解质液(GES)时胃排空和胃血流量的影响。方法:雄性SD大鼠随机分为口服GES组(GES,n=16);失血+口服GES+电针非经非穴组(H+GES/SEA,n=16)和失血+口服GES+电针足三里穴组(H+GES/EA,n=16)。大鼠用氯胺酮-速眠新Ⅱ肌肉注射复合麻醉后,行右侧颈动脉插管,按全身血容量的40%分两次间隔15 min放血制作失血性休克模型。于失血后0.5h及1h分两次给予2倍失血量的GES灌胃。需电针治疗的大鼠用自制布袋固定后,于第二次灌胃后在清醒状态下电针足三里穴(频率2～100Hz,强度2～3mA,时间30min)。非经非穴组于失血口服GES后刺激非经非穴(足三里穴外侧旁开0.5cm,频率2～100Hz,强度2～3mA,时间30min)。用激光多谱勒血流仪测定失血后2 h和4 h胃组织血流量,采用酚红法测定胃排空率。结果:失血后2 h和4 h,H+GES/SEA组胃排空率分别为(42.2±7.8)%和(62.4±11.4)%,显著低于GES组(86.8±7.2)%和(92.4±12.5)%(P均<0.01);胃血流量分别为(80.091±42.200)U和(94.641±9.442)U,显著低于GES组(161.760±38.277)U和(146.010±17.512)U(P均<0.01)。H+GES/EA组失血后2 h和4 h胃排空率比H+GES/SEA组提高34.0%和18.0%;胃血流量增加17.0%和12.6%(P<0.05或P<0.01)。结论:电针足三里穴能显著增加大鼠失血性休克早期胃排空率和胃血流量,提高口服液体复苏的效果。Objective：To investigate the effects of electro-acupuncture （EA） at Zunsanli point （ST36,足三里穴） on gastric emptying and gastric blood flow in oral resuscitation of early shock with glucose electrolyte solution （GES） in rats with 40% blood loss. Metbods：SD rats were randomly divided into three groups： oral GES without hemorrhage group （GES group）, hemorrhage resuscitated with oral GES ＋ sham-EA group （H＋ GES/SEA）, and hemorrhage resuscitated with oral GES ＋ EA group （GES/EA group）. About 40% of total blood volume was drawn from carotid artery of rats. GES with its volume two times of that of blood loss was given twice into the stomach with an interval of 30 minutes after the blood loss. Bilateral Zusanli points were eletroacupunctured with constant voltage （2--3 mA,2--100 Hz） to rats without anesthesia for 30 minutes after the second intragastric fluid administration. The gastric emptying rate with method of phenol red emptying and gastric blood flow by laser Doppler were determined at 2 hours and 4 hours after hemorrhage. Results：It was showed that the gastric emptying and gastric blood flow at 2 hours and 4 hours in H＋GES/SEA group respectively were （42.2±7.8）% and （62.4±11.4） %, （80. 091±42. 200）and（94. 641±9. 442） ,which were dramatically decreased compared to those in GES group [（86.8±7.2）% and（92.4±12.5）%,（161. 760±38. 277）U and（146. 010±17. 512）U, all P〈0. 01]. EA resulted in 34.0% and 18.0% of promotion of gastric emptying at 2 hours and 4 hours after bleeding in H＋ GES/EA group compared to those in H＋GES/SEA group. EA also increased 17.0% and 12.6% of gastric blood flow in H＋GES/EA group （P〈0.05 or P〈0.01）. Conclusion：The results indicate that electro-acupuncture at Zusanli points has significant effect on improving gastric emptying and gastric blood flow in the resuscitation of hemorrhagic shock with oral rehydration salt solution.

关 键 词：电针足三里穴 失血 口服补液 胃排空 局部血流 

分 类 号：R224.2[医药卫生—中医基础理论] R245.97[医药卫生—中医学]