英夫利西单抗对溃疡性结肠炎模型大鼠的治疗作用  

Therapeutic effect of infliximab on ulcerative colitis model of rat

作　　者：肖珑[1] 万军[2] 刘士敬[1] 史文丽[1] 宫嫚[1] 王昌正[2] 黄海力[2] 

机构地区：[1]解放军302医院感染16科,北京100039 [2]解放军总医院南楼消化科,北京100853

出　　处：《军医进修学院学报》2009年第5期703-705,共3页Academic Journal of Pla Postgraduate Medical School

摘　　要：目的观察英夫利西单抗对溃疡性结肠炎(UC)模型大鼠的治疗机制。方法选取48只成年Wistar大鼠随机分成正常组、英夫利西单抗低、中、高剂量组、美沙拉秦组和模型组,每组8只,建立大鼠UC模型,英夫利西单抗低剂量给予2.5mg/kg,中剂量给予5mg/kg,高剂量给予10mg/kg,皮下注射,第1、7日给药,美沙拉秦组给予100mg/kg,每日给药。造模两周后,眼球取血1.5mL,ELISA检测各组大鼠TNF-α、IL-8。结果UC模型大鼠TNF-α、IL-8含量显著降低(P<0.05)。结论英夫利西单抗对UC模型大鼠有较好的治疗作用。Objective To observe the effect of infliximab on ulcerative colitis （UC） model of rat. Methods Forty-eight healthy adult Wistar rats were selected and divided randomly into normal group, low infliximab dose group, medium infliximab dose group, high infliximab dose group mesalazine group and model group （n=8）. A UC model of rat was established by subcutaneous injection of infliximab at the doses of 2.5mg/kg, 5mg/kg and 10mg/kg, respectively, on days 1 and 7. Rats in mesalazine group received intragastric mesalazine, 100mg/kg body weight, once a day. Two weeks after establishment of the model, 1.5mL blood was collected from fossa orbitalis to detect TNF-α and IL-8 by ELISA. Results The TNF-α and IL-8 expression levels were significantly decreased in the UC model. Conclusion Infliximab has rather good therapeutic effects in UC model of rats.

关 键 词：英夫利西单抗 结肠炎 溃疡性 大鼠 

分 类 号：R57[医药卫生—消化系统]