苦杏仁甙对博莱霉素大鼠肺纤维化Ⅰ、Ⅲ型胶原表达的影响  

Effect of amygdlin on typeⅠand Ⅲ collagen expression in rats with pulmonary fibrosis induced by bleomycin

作　　者：杜海科[1] 宋福成[2] 周欣[2] 李贺[2] 张健鹏[3] 

机构地区：[1]解放军总医院,北京100853 [2]武警医学院附属医院心内科,天津300162 [3]武警总医院呼吸科,北京100039

出　　处：《军医进修学院学报》2009年第5期712-714,717,共4页Academic Journal of Pla Postgraduate Medical School

基　　金：武警医学院院级课题(WY2007-1)

摘　　要：目的探讨苦杏仁甙对博莱霉素大鼠肺纤维化Ⅰ、Ⅲ型胶原表达的影响。方法采用气管暴露法建立大鼠博莱霉素肺纤维化模型,制作石蜡切片,HE染色光镜观察,天狼猩红染色结合偏振光显微镜观察肺组织Ⅰ、Ⅲ型胶原的表达,图像分析系统对胶原定量分析。结果建模后第7天、第14天和第28天时,博莱霉素组大鼠肺组织Ⅰ、Ⅲ型胶原面积百分比均高于对照组(P<0.01);与博莱霉素组大鼠比较,第28天时苦杏仁甙组大鼠肺组织Ⅰ型胶原面积百分比降低(P<0.01);3个时间点苦杏仁甙组大鼠Ⅲ型胶原面积百分比均低于博莱霉素组(P<0.01)。结论苦杏仁甙通过抑制Ⅰ、Ⅲ型胶原的形成,可有效地减缓博莱霉素诱导的大鼠肺纤维化形成,提示该药有可能用于人类肺纤维化的防治。Objective To evaluate the effect of amygdlin on expression of type Ⅰ and Ⅲ collagen in an animal model of pulmonary fibrosis induced by bleomycin. Methods An animal model was established by direct tracheal instillation of bleomycin into rat lungs at tracheostomy. Paraffin-embedded lung sections were stained with H＆E and Sirius red. Histologic changes in the lung were evaluated using light microscopy. Expression of type Ⅰ and Ⅲ collagen was detected by polarized light microscopy and quantified by Image-Pro Plus. Results The percentage of type Ⅰ and Ⅲ collagen positive area in rat lungs was significantly higher in bleomycin group than in control group after 7, 14 and 28 days after establishment of the animal model（P〈0.01）. The percentage of type I collagen positive area in rat lungs was reduced in amygdlin group 28 days after establishment of the animal model compared with the bleomycin group（P〈0.01）. The percentage of type llI collagen was lower in amygdlin group than in bleomycin at the three time points（P〈0.01）. Conclusion Amygdlin reduce bleomycin-induced increase of type Ⅰ and Ⅲ collagen in rat lungs, suggesting that amygdlin can be used in treatment of pulmonary fibrosis in human beings.

关 键 词：苦杏仁甙 博莱霉素 大鼠 肺 胶原Ⅰ型 胶原Ⅲ型 

分 类 号：R36[医药卫生—病理学]