壳聚糖温敏凝胶对人牙龈成纤维细胞的毒性研究  

Cytotoxicity of chitosan thermosensitive hydrogel to human gingival fibroblasts in vitro

作　　者：王立妍[1] 彭伟[2] 穆玉[2] 

机构地区：[1]唐山市开滦医疗集团医院,河北唐山063000 [2]华北煤炭医学院口腔系,河北唐山063000

出　　处：《军医进修学院学报》2009年第5期715-717,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：河北省科技攻关项目(07275524)~~

摘　　要：目的了解壳聚糖温敏凝胶对人牙龈成纤维细胞的毒性作用,为其治疗牙周病提供依据。方法人牙龈成纤维细胞在不同浓度的壳聚糖温敏凝胶中体外培养24h、48h、72h、96h,用MTT法测定细胞相对增殖率(RGR),判断细胞毒性的级别。结果各组细胞不同时间点相对增殖率均在96%～144%之间,各浓度的壳聚糖水凝胶材料浸提液的细胞毒性均为0级或1级,完全符合生物材料的安全评价标准。结论壳聚糖温敏凝胶对人牙龈成纤维细胞无毒性,用于牙周病局部治疗具有进一步的研究价值。Objective To study the cytotoxicity of chitosan thermosensitive hydrogel to human gingival fibroblasts （HGF） for its application in the treatment of periodontal diseases. Methods Human gingival fibroblasts were cultured in a medium containing chitosan thermosensitive hydrogel at different concentrations for 24, 48, 72, and 96h, respectively. The relative growth rate （RGR） of HGF was determined by MTT assay. Results The relative growth rate of HGF was over 90% after cultured for different periods of time. The cytotoxicity grade of different concentrations of chitosan thermosensitive hydrogel was 0 and 1. Conclusion RGR have no cytotoxicity to HGF. Local application of chitosan thermosensitive hydrogel in the treatment of periodontal diseases is feasible.

关 键 词：牙龈 成纤维细胞 牙周病学 水凝胶 壳聚糖 细胞毒性试验 免疫 

分 类 号：R781.4[医药卫生—口腔医学]