海南黑山羊生长激素(GH)基因第5外显子单核苷酸多态性分析  

Single Nucleotide Polymorphism Analysis of Hormone(GH) Growth Gene in Hainan Black Goat

作　　者：管松[1] 李琼[1] 周汉林[1] 侯冠彧[1] 曾鸿普[1] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所,海南儋州571737

出　　处：《热带农业工程》2009年第3期54-58,共5页Tropical Agricultural Engineering

基　　金：海南省自然科学基金(No.4282)

摘　　要：利用PCR-SSCP与测序相结合的方法,分析了海南黑山羊GH基因第5外显子的多态性。SSCP结果出现3种基因型,分别定义为CC、CG、GG,3种基因型频率分别为:21.3、73.2和5.5%,C、G等位基因频率分别为57.9和42.1%。以C等位基因为优势基因,Hardy-Weinberg平衡卡方适合性检验结果表明,GH基因第5外显子在此群体中处于Hardy-Weinberg平衡状态(X2=0.0022,P>0.05),该位点群体遗传结构及多态指标分析结果显示该位点表现为高度多态。CC和GG基因型进行测序结果发现,GH基因exon5第75位点发生G→C转换,将核苷酸序列翻译成氨基酸序列进行同源性比较,结果exon5第75位点G→C转换没有引起编码的氨基酸转换,属于沉默突变。The polymorphism of GH gene exon 5 of Hainan black goats was analysed by PCR-SSCP and sequencing. The SSCP result showed that 3 genotypes were discovered and defined as CC, CG and GG respectively, with their respective frequencies being 21.3 %, 73.2 % and 5.5 %, and the C and G allele frequencies 57.9 % and 42.1%, respectively. G allele gene is dominant. The chi-square goodness of fit test for Hardy-Weinberg equilibrium showed that the GH gene exon 5 in this population lied in the Hardy-Weinberg equilibrium （X^2=0.002 2, P〉0.05）, and analysis of the population genetic structure and polymorphism at this site showed that the site of GH gene exon 5 was highly polymorphic. Sequencing of CC and GG genotypes showed that G→C transversion occurred at position 75 of exon 5 of the GH gene. The nucleotide sequence was translated into the amino acid sequence and compared in terms of homology, and the results showed that the G→C transversion at position 75 of exon 5 failed to cause conversion of the coded amino acids, which was called silent mutation.

关 键 词：海南黑山羊 GH基因 单核苷酸多态性 氨基酸 

分 类 号：S82[农业科学—畜牧学]