局灶性脑缺血再灌注大鼠海马CA1区神经元凋亡研究  

The Experimental Study on the Apoptosis of the Hippocampal CA1 Subregion after Focal Cerebral Ischemia Reperfusion in Rats

作　　者：王大力[1] 张江[1] 彭延波[1] 赵晓晶[1] 常莉莎[1] 

机构地区：[1]华北煤炭医学院附属医院神经内科二病区,河北唐山063000

出　　处：《中国神经免疫学和神经病学杂志》2009年第5期337-339,343,共4页Chinese Journal of Neuroimmunology and Neurology

摘　　要：目的观察大鼠局灶性脑缺血再灌注(ischemiareperfusion,I/R)损伤后海马CA1区神经元凋亡、TUNEL阳性细胞变化,以及凋亡相关蛋白Bcl-2与Bax蛋白的表达情况。方法将健康雄性SD(Sprague-Dawley)大鼠随机分为假手术组和I/R组,每组再分为缺血再灌注后3、6、12、24、48、72 h亚组。应用免疫组化方法检测再灌注后不同时间点大鼠海马CA1区神经元凋亡基因Bcl-2和Bax蛋白的表达及Bcl-2/Bax比值变化,采用原位细胞凋亡检测(TUNEL)技术检测凋亡阳性细胞数。结果各组非缺血侧相应区域神经元胞质中Bcl 2均有微弱表达。I/R组缺血侧海马CA1区于再灌注3 h开始出现Bcl-2和Bax蛋白微弱表达,随再灌注时间延长神经元内Bcl-2表达逐渐增强,再灌注24 h后Bcl-2表达达高峰,假手术组与I/R组比较差异有统计学意义(P<0.05)。结论 I/R损伤后海马CA1区神经元不仅存在变性坏死,还存在明显的细胞凋亡且细胞凋亡在大鼠I/R损伤中发挥重要作用;I/R可诱导海马CA1区细胞凋亡基因Bcl 2和Bax蛋白表达,且其表达呈一定规律。Objective To observe the neuronal apoptosis, the changes of tunel positive cells, the expression of Bcl 2, Bax proteins after local cerebral ischemia reperfusion in the hippocampal CA1 subregion of rats. Methods Healthy male SD （Sprague-Dawley） rats were used and were taken randomly into two groups： （1）SO （sham operation） group; （2）I/R（isehemia reperfusion） group. Then each group was taken into 3 h, 6 h, 12 h, 24 h, 48 h, 72 h subgroup according to the different reperfusion time, totally six groups. Bcl-2, Bax proteins and values of Bcl-2/Bax were checked by immunohistoehemieal staining methods in the hippocampal CA1 subregion. Apoptosis of cells was observed by TUNEL staining methods. Results There was little Bax expression in the contralateral hippocampal CA1 subregion which shows no ischemia in each group. In the ischemie hippocampal CA1 subregion of the I/R group, the expression of Bax was still little 3 hour after reperfusion. The expression of Bax began increasing 6 hour after reperfusion, it peaked at 24 h. The expression decreased significantly compared with that in I/R group, there were significant differences among these groups （P〈0.05）. Conclusions After cerebral reperfusion injury, there is not only denaturation and necrotic neurons, but also apoptosis. Maybe apoptosis was the main died style in the hippocampus CA1 subregion. Cerebral ischemia reperfusion can induce the expression of Bcl-2 and Bax. Cell apoptosis plays an important role in the cerebral ischemia reperfusion injury. The expression of Bcl-2 and Bax has some rule in hippocampal CA1 neurons in focal cerebral ischemia reperfusion in rats.

关 键 词：脑缺血再灌注 Bcl-2 BAX 凋亡 

分 类 号：R743.31[医药卫生—神经病学与精神病学]