全营养液对急性肝功能衰竭大鼠脂代谢的影响  

Effect of total nutrient admixture on lipid metabolism in acute hepatic failure rats

作　　者：齐玉梅[1] 张明[1] 陈亚军[1] 许晋[1] 杨辉[1] 

机构地区：[1]天津市第三中心医院营养科 300170

出　　处：《中华临床营养杂志》2009年第1期41-44,I0005,共5页Chinese Journal of Clinical Nutrition

摘　　要：目的探讨全营养液对急性肝功能衰竭大鼠脂代谢的影响。方法将56只急性肝功能衰竭模型大鼠随机分为普通饲料组、无氮饲料组、无脂肪营养液组和全营养液组。采用D-半乳糖胺诱导法复制急性肝功能衰竭大鼠模型。同时用6只健康雄性Wistar大鼠作为对照组。全营养液组大鼠每日输注的营养液提供能量221．75kJ（1kcal=4．184kJ），氮0．365g，氮热比为1：145，氨基酸2．28g，其中支链氨基酸0．70g，葡萄糖7．85g，脂肪1．25g。颈外静脉插管穿刺手术后10天取血，监测血糖、血脂、肝肾功能。结果全营养液组的血糖水平显著高于无脂肪营养液组（P〈0．05）。4组中无脂肪营养液组的血清甘油三酯和胆固醇水平均最高，甘油三酯水平显著高于普通饲料组和无氮饲料组（P〈0．05）；全营养液组的血清甘油三酯和胆固醇水平显著高于普通饲料组（P〈0．05）。全营养液组的丙氨酰氨基转移酶、碱性磷酸酶、总胆红素、血尿素氮水平均低于无脂肪营养液组（P〈0．05）。结论本研究采用的全营养液的结构比例适合于急性肝功能衰竭大鼠的代谢需要，能促进机体的合成代谢，有利于逆转大鼠的急性肝功能衰竭。Objective To study the effect of total nutrient admixture （TNA） on lipid metabolism in rats with acute hepatic failure （AHF）. Methods Fifty-six male Wistar rats were randomly divided into four groups： normal diet group, nitrogen-free diet group, fat-free nutrient admixture group, and TNA group. All rats were injected with D-galac- tosamine to induce AHF model. Meanwhile, 6 healthy Wistar rats were used as control group. TNA provided energy 221.75 kJ （1 kcal=4. 184 kJ）, nitrogen 0.365 g, nitrogen to energy ratio 1：145, amino acids 2.28 g （including branched-chain amino acid O. 70 g）, glucose 7. 85 g, and fat 1.25 g on a daily basis. After 10 days of the operation of Jugular vein puncture tube, blood glucose, blood lipid, liver and renal function were determined. Results The blood glucose level was significantly higher in TNA group than that in fat-free nutrient admixture group （P 〈 0. 05 ）. The levels of serum triglyceride and cholesterol were highest in fat-free nutrition admixture group, and triglyceride level was signif- icantly higher than that in normal diet group and nitrogen-free diet group （P 〈 0. 05 ）. The levels of serum triglyceride and cholesterol were significantly higher in TNA group than those in normal diet group （P 〈0. 05）. The levels of ala- nine aminotransferase, alkaline phesphatase, total bilirubin and blood urea nitrogen were lower in TNA group than those in fat-free nutrient admixture group （P 〈 0. 05 ）. Conclusion The nutritional proportion of TNA is suitable for the metabolism disorder of hepatic failure, and therefore TNA can reasonably promote the anabolism and reverse the deterioration of hepatic failure in rats.

关 键 词：全营养液 急性肝功能衰竭 脂代谢 

分 类 号：R575[医药卫生—消化系统]