c-myc RNA干扰增强肺腺癌细胞对吉西他滨的敏感性  

c-myc RNA interference sensitizes lung adenocarcinoma cells to gemcitabine

作　　者：刘黎明[1] 张永[1] 胡俊峰[1] 

机构地区：[1]蚌埠医学院第一附属医院呼吸病科,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2009年第9期765-768,共4页Journal of Bengbu Medical College

摘　　要：目的:探讨c-mycRNA干扰后肺腺癌细胞A549对吉西他滨敏感性的改变。方法:构建c-myc特异RNA小干扰(siRNA)的表达载体,转染A549细胞,G418筛选出稳定表达c-myc特异siRNA的细胞株,荧光实时定量RT-PCR和免疫印迹检测c-myc基因表达水平。吉西他滨作用于干扰有效A549细胞,分为GE+siRNA组、GE组、siRNA组和空白对照组。每组12例,四甲基偶氮唑蓝法检测吸光度,流式细胞仪测凋亡率。结果:成功构建c-myc-siRNA表达载体。c-myc基因mRNA和蛋白表达下降71.9%和85.6%。吉西他滨作用于c-myc-siRNA细胞其吸光度在各时间点较单用吉西他滨组、单用c-myc-siRNA组以及对照组均下降(P<0.01),凋亡率增加(P<0.01)。结论:c-mycRNA干扰后A549细胞的增殖减慢,对吉西他滨的敏感性增加。Objective：To investigate the sensitivity of lung adenocarcinoma cells to gemcitabine （GE）after c-myc RNA interference. Methods：The c-myc-siRNA expression vector was constructed and confirmed by sequencing,and its expression vector was transfected into A549 cells. C,418 （geneticin）was used for selecting the cell line which expressed c-myc-siRNA stably. The level of c-myc was tested by FQ RT-PCR and western blot. The transfected cells were treated with gemcitabine,which were then divide into GE ＋ siRNA group, GE group,siRNA group and control group, with 12 cases in each. The cell proliferation and apoptosis were assayed by tetrazolium bromide colorimetry and flow cyelometry, respectively. Results：c-myc-siRNA expression vector was constructed and transfected into A549 cells successfully. The c-myc mRNA and protein expression was effectively reduced（71.9% and 85.6% respectively）. The cells treated with c-myc-siRNA and gemcitabine had a lower absorbance value at each time point than that of simple gemcitabine group, simple c-myc-siRNA group or control group （ P 〈 0.01 ）, and the apoptosis rate was also decreased （ P 〈 0.01 ）. Conclusions ： The c-myc RNA interference can enhance the sensitivity of lung adenocarcinoma A549 cells to gemeitabine.

关 键 词：肺肿瘤 吉西他滨 核糖核酸干扰 细胞株 

分 类 号：R734.2[医药卫生—肿瘤]