美国白蛾核型多角体病毒多角体蛋白的SDS-PAGE分析  

SDS-PAGE Analysis on Protein of Hyphantria cunea Nuclear Polyhedrosis Virus

作　　者：薛建杰[1,2] 曲良建[3] 王玉珠[3] 张永安[3] 唐明[1] 杨唯一[1,2] 

机构地区：[1]西北农林科技大学林学院,陕西杨凌712100 [2]中国林业科学研究院森林生态环境与保护研究所,国家林业局森林保护学重点实验室,北京100091 [3]中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室,北京100091

出　　处：《林业科学研究》2009年第5期728-731,共4页Forest Research

基　　金：国家自然科学基金(30671688)资助;国家“十一五”科技支撑计划(2006BAD08A1202)

摘　　要：Hyphantria cuea nucleopolyhedrovirus(HcNPV) and several other nucleopolyhedrosis was extracted by differential centrifugation,and purified with sucrose gradient centrifugation method.Polyhedrin was gotten and analysed by SDS-PAGE.It indicated that most of those proteins had 32 KD protein band,and with 64 KD protein,and might be the main protein dimer with the structure and found Hyphantria cunea nuclear polyhedrosis virus protein in 18 KD department had a specific band.Hyphantria cuea nucleopolyhedrovirus （HcNPV） and several other nucleopolyhedrosis was extracted by differential centrifugation, and purified with sucrose gradient centrifugation method. Polyhedrin was gotten and analysed by SDS - PAGE. It indicated that most of those proteins had 32 KD protein band, and with 64 KD protein, and might be the main protein dimer with the structure and found Hyphantria cunea nuclear polyhedrosis virus protein in 18 KD department had a specific band.

关 键 词：HCNPV 核型多角体病毒 多角体蛋白 SDS-PAGE 32KD蛋白 

分 类 号：S476.13[农业科学—农业昆虫与害虫防治] S763.42[农业科学—植物保护]