TRAIL受体在儿童急性白血病中的表达及其临床意义  

Expression of TRAIL Receptors and Its Significance in Children with Acute Leukemia

作　　者：张小玲[1] 胡群[1] 宋艳清[1] 陶红芳[1] 侯燕[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院儿科,武汉430030

出　　处：《华中科技大学学报（医学版）》2009年第4期538-540,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.39970778)

摘　　要：目的探讨肿瘤坏死因子相关凋亡诱导配体（TRAIL）的4种受体TR1-R4在儿童急性白血病（AL）中的表达,及其与临床分型和预后的关系。方法应用半定量逆转录聚合酶链反应（RT-PCR）测定22例AL患儿（AL组）和10例骨髓正常的非恶性血液病患儿（对照组）TR1-R4 mRNA的表达情况。结果AL组死亡受体DR4（TR1）和DR5（TR2）mRNA的表达明显高于对照组（P〈0.01）;而AL组蒙骗受体DcR1（TR3）和DcR2（TR4）mRNA的表达明显低于对照组（P〈0.05）。AL组与对照组均表达DR5,但AL组内DR5表达相对量明显高于DR4,差异具有统计学意义（P〈0.05）。AL患儿不同亚型之间TRAIL受体表达差异无统计学意义。结论TRAIL受体TR1-R4在儿童急性白血病中表达有明显差异性,TRAIL及其受体在白血病细胞凋亡中起重要作用。Objective To investigate the expression of TRAIL receptors（TR1―R4） and its correlation with clinical classification and prognosis of acute leukemia（AL）.Methods Reverse transcription polymerase chain reaction（RT-PCR） was used to detect the expression of TRAIL receptors in leukemic cells of 22 children with acute leukemia（AL）.Ten cases of children with no-malignant blood diseases served as control group.Results The mRNA expression of DR4 and DR5 in AL group was dramatically higher than that in control group（P〈0.05）.However,the mRNA expression of DcR1 and DcR2 in AL group was significantly lower than in control group（P〈0.05）.DR5 was expressed in both AL group and control group,but its mRNA relative amount was markedly higher than that of DR4 in AL group.No significant difference in the expression of TR1-R4 was found among different clinical sub-types.Conclusion There were significant differences in the expression of TRAIL receptors TR1-R4 among children with acute leukemia.TRAIL and its receptors play an important role in apoptosis of leukemic cells.

关 键 词：肿瘤坏死因子相关凋亡诱导配体 基因表达 急性白血病 儿童 

分 类 号：R733.71[医药卫生—肿瘤]