ISSR分子标记及其在水产生物遗传学中的应用  

ISSR Mark and Its Application in the Genetics of Aquatic Organisms

作　　者：叶丽平[1,2] 劳沈颖[1] 吕耀平[1] 杨燕波[1] 

机构地区：[1]丽水学院化学与生命科学学院,浙江丽水323000 [2]温州市第二十一中,浙江温州325000

出　　处：《丽水学院学报》2009年第5期17-22,共6页Journal of Lishui University

基　　金：浙江省教育厅项目(20070552);丽水市科技局项目(2007kjh2005);丽水市人才工作专项资金项目资助(LSRZB2008013)

摘　　要：ISSR是一种基于微卫星序列发展起来的新的分子标记,具有简便、稳定、DNA多态性高等优点。ISSR标记呈孟德尔式遗传,大部分ISSR标记为显性标记。综合有关文献就ISSR的原理、操作及其在水产生物品种鉴定、亲缘关系分析、遗传多样性检测、遗传图谱构建和分子标记辅助育种等方面的应用和进展进行了简要的阐述。Inter-simple sequence repeat （ISSR）is a microsatellite-based technique being used for genetic and breeding studies as a new molecular mark.ISSR marks are a quick,simple and reliable method and may also generate greater DNA polymorphism.ISSR marks are inherited and segregated in Mendelian fashion and most of which behave as dominant marks.This paper emphasizes on the principle and procedures of ISSR and its application to variety identification,phylogenetic analysis, genetic diversity detection,genetic map construction and mark assisted selection in aquatic organisms.

关 键 词：ISSR 水产生物 遗传多样性 分子标记辅助育种 

分 类 号：Q24[生物学—细胞生物学]