脂质体槲皮素对Eca109/9706细胞增殖及血管内皮生长因子和c-met表达的影响  

Effect of proliferation,c-met and vascular endothelial growth factor expression in the Eca109/9706 cells by liposomal quercetin

作　　者：裴迎新[1] 杜晓燕[1] 李金萍[1] 高涵昌[1] 王莎莎[1] 

机构地区：[1]郑州大学公共卫生学院营养与食品卫生学教研室,郑州450001

出　　处：《卫生研究》2009年第5期599-602,共4页Journal of Hygiene Research

摘　　要：目的检测不同脂质体槲皮素对食管癌Eca109/9706细胞增殖及c-met和血管内皮生长因子(VEGF)表达的影响。方法MTT实验检测脂质体槲皮素和非脂质体槲皮素对Eca109/9706细胞生长的抑制率;免疫组织化学染色及免疫印迹检测脂质体槲皮素处理48h后Eca109/9706细胞中c-met,VEGF表达的改变。结果MTT检测表明,Eca109/9706的细胞生长抑制率依次为脂质体槲皮素(LQ2)组>脂质体槲皮素(LQ1)组>非脂质体槲皮素(nLQ)组>对照组(P<0.05);在人食管癌Eca109/9706细胞中,c-met及VEGF的免疫组织化学图像扫描灰度均值从高至低依次为:对照组>nLQ组>LQ1组>LQ2组(P<0.05)。c-met和VEGF的免疫印迹信号数值为各主带扫描灰度均值与各β-actin扫描灰度均值的比值,从高至低依次为:对照组>nLQ组>LQ1组>LQ2组(P<0.05)。结论脂质体槲皮素可抑制Eca109/9706细胞的增殖,其作用可能与降低c-met及VEGF的高表达有关。Objective To explore the effect of cell proliferation,c-met and vascular endothelial growth factor（VEGF） expression by liposomal quercetin in the Eca109/9706 cells induced by liposomal quercetin. Methods The suppressing rate of cultured Eca109/9706 cells was detected by MTT assay. After 48-hour-exposure to liposomal quercetin, the expression and cellular localization of c-met and VEGF in Eca109/9706 cells were examined by using immunohistochemistry assay and Western blotting. Results The suppressing rate of Eca109/ 9706 ceils was in the order of LQ2 group 〉 LQ1 group 〉 nLQ group 〉 control group （P 〈 0.05） . The protein expression of e-met and VEGF was found in Eca109/9706 cells eytoplasma as well as in nuclei by using immunobistochemistry assay. The Immuno-reaction intensity of c-met and VEGF was in the order of control group 〉 nLQ group 〉 LQ1 group 〉 LQ2 group （P 〈 0.05）. The western blotting showed the intensity of immunoblotting signals for c-met-IR and VEGF-IR was in the order of control group 〉 nLQ group 〉 LQ1 group 〉 LQ2 group （ P 〈 0.05 ）. Conclusion The liposomal quercetin could suppress the proliferation of culture Eca109/ 9706 cells, which was about to be related with the suppression high expression of c-met and VEGF.

关 键 词：脂质体槲皮素 ECA 109/9706 细胞增殖 血管内皮生长因子 C-MET 食管肿瘤 

分 类 号：R735.1[医药卫生—肿瘤] Q562[医药卫生—临床医学]