γ-环糊精-中性红包合物与鲱鱼精DNA的相互作用  

Interaction between γ-Cyclodextrin-Neutral Red Inclusion Complex and Herring Sperm DNA

作　　者：赵娜[1] 王兴明[1] 潘海专[1] 胡亚敏[1] 熊远纳[1] 丁立生[2] 

机构地区：[1]西南科技大学材料科学与工程学院,四川绵阳621010 [2]中国科学院成都生物研究所,四川成都610041

出　　处：《武汉大学学报（理学版）》2009年第5期526-530,共5页Journal of Wuhan University:Natural Science Edition

基　　金：国家自然科学基金资助项目(30572254)

摘　　要：采用紫外-可见、荧光光谱等研究手段,确定了γ-环糊精-中性红包合物(γ-CD-NR)与鲱鱼精DNA之间存在嵌插和静电两种作用方式.用摩尔比法和双倒数法确定了γ-CD与NR的包合比nγ-CD∶nNR=1∶1,包合常数Kf=2.08×103L.mol-1.γ-CD-NR包合物与鲱鱼精DNA的结合比nγ-CD-NR∶nDNA=4∶1,结合常数K■25℃=2.11×106L.mol-1.化学热力学研究显示γ-CD-NR包合物与鲱鱼精DNA的结合为熵驱动.以吖啶橙(AO)作荧光探针,研究发现γ-CD-NR包合物和AO在与DNA作用时存在竞争作用.The mechanism of interaction between γ-cyclodextrin-neutral red （γ-CD-NR） inclusion complex and herring sperm DNA was studied by UV-Vis and fluorescence spectra. The results suggest that the intercalation and electrostatic binding should be the two major modes for the interaction between γ-CD-NR and DNA. It were confirmed by molar ratio method and double reciprocal method that the binding ratio of nγ-CD ： nNR = 1 ： 1 and the binding constant of Kf = 2.08 × 10^3 L· mol^-1. While the binding ratio of nγ-cD-NR ： nDNA =4：1 and the binding constant of K25℃ =2.11× 10^6L·mol^-1. Thermodynamic function computation demonstrated that entropy （△rSm ） is the primary driving power of the interaction between γ-CD-NR and DNA. The results suggest that there are competitive action between γ-CD-NR inclusion complex and AO when they interact with DNA.

关 键 词：Γ-环糊精 中性红 包合物 鲱鱼精DNA 作用方式 

分 类 号：O629.74[理学—有机化学]