棉花内生菌筛选纯化及抑菌效果研究  

The Research of Selection、Purification and Restain Fungus Effection of Endophytic Fungus of Cotton

作　　者：孔庆军[1] 王莹[1] 葛彬[1] 

机构地区：[1]石河子大学生命科学院,新疆石河子832003

出　　处：《安徽农学通报》2009年第17期79-81,共3页Anhui Agricultural Science Bulletin

基　　金：石河子大学高层次人才项目(O7002-500002O62001)资助

摘　　要：内生菌是一类新的微生物资源,能够产生多种生物活性物质,具有重要的研究价值,开发前景广阔[8]。本文对新疆地区健康棉花根部[7-9]幼叶进行表面消毒灭菌、分离、纯化、鉴定的研究,初步了解棉花内90%的内生菌都为芽孢杆菌[1]。参照《常见细菌鉴定手册》对菌株的属种及其形态、培养性状和生理生化特性进行鉴定,试验得出芽孢杆菌的分离频率最高,为优势种群。平板对峙试验表明,芽孢杆菌对两种植物病原真菌有抑制作用。Endophytes are new microorganism resoures which have the sbility to produce various bioactive substances, which have great research value and a bright prospect in exploitation. Throughing the research of surface disinfection, sterilization, isolation,identification and screening to Health region of Xinjiang cotton root, we found that Bacillus has been identified in the Bacillus carried out under sterile conditions purification training. In the light of ＂common bacteria identified manual＂ strain of the species and its shape, training Characters and physiological and biochemical characteristics were identified, the pilot that the separation of Bacillus highest frequency for the dominant population. Endophytic bacteria showed activities to inhibit two pathogenic fungi in vitr.

关 键 词：棉花内生菌 芽孢杆菌 筛选 纯化 抑菌 

分 类 号：S562[农业科学—作物学]