应用单细胞微凝胶电泳技术研究细胞DNA损伤的原理与方法  

PRINCIPLE AND METHOD OF THE SINGLE CELL MICROGEL ELECTOPHORESIS (SCGE) TECHNIQUE TO STUDY DNA DAMAGE IN MAMMALIAN CELLS

作　　者：孟紫强[1,2] 张连珍[1,2] 

机构地区：[1]山西大学生命科学系环境生物毒理学研究室 [2]中国辐射防护研究院二所

出　　处：《癌变．畸变．突变》1998年第6期377-382,共6页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：本文对单细胞微凝胶电泳（ＳＣＧＥ）技术的操作过程，技术原理以及实验操作过程中应注意的事项，进行了详细介绍和讨论；并应用ＳＣＧＥ技术研究了γ线照射对人血淋巴细胞ＤＮＡ的损伤效应。结果表明，γ线照射能引起细胞ＤＮＡ迁移长度增加，有显著的剂量效应关系。In this paper, the SCGE technique, its principle and noticeable details in the technique were described and misscussed; and effects of DNA damage of human blood lymphocytes induced by γ rays radiation were studied using this technique. It was shown that γ rays caused the increase of DNA migration length in the lymphocytes in the dose dependent manner.

关 键 词：DNA损伤 淋巴细胞 电离辐射 SCGE 

分 类 号：R361.3[医药卫生—病理学]