2-(9-蒽基)-2-甲氧基乙酸乙酯的高效液相色谱手性拆分  

Chiral Resolutions of Ethyl 2-(9-Anthryl)-2-methoxyacetate by High-performance Liquid Chromatography

作　　者：郑之祥[1] 张振国[1] 李宝林[1] 

机构地区：[1]药用资源与天然药物化学教育部重点实验室,陕西师范大学化学与材料科学学院,陕西西安710062

出　　处：《分析科学学报》2009年第5期590-592,共3页Journal of Analytical Science

摘　　要：以键合在5μm硅胶上的纤维素-三(二甲基苯基氨基甲酸酯)为色谱柱的手性固定相,采用高效液相色谱(HPLC)法对外消旋的2-(9-蒽基)-2-甲氧基乙酸乙酯进行了手性拆分。对影响2-(9-蒽基)-2-甲氧基乙酸乙酯拆分的三个重要因素:流动相组成、流速、色谱柱温度进行了研究。实验结果表明,在流动相组成为正已烷-异丙醇(94/6,V/V),流速1.0 mL/min,柱温20℃的条件下,2-(9-蒽基)-2-甲氧基乙酸乙酯对映体得到很好的分离,分离度为3.63。The racemic ethyl 2-（9-anthryl）-2-methoxyacetate was resoluted by high-performance liquid chromatography with a cellulose-tris-3,5-dimethylphenyl carbamate-based chiral column successfully.Various factors which can affect the resolution of the compound such as mobile phase,flow rate and column temperature were investigated.Under the conditions of 6% of 2-propanol in n-hexane（V/V） as mobile phase,flow rate of 1.0 mL/min and column temperature of 20℃,（±）-9AMA were successfully separated at Rs 3.63.

关 键 词：2-(9-蒽基)-2-甲氧基乙酸乙酯 高效液相色谱法 手性分离 

分 类 号：O657.72[理学—分析化学]