混苯接触工人外周血淋巴细胞染色体畸变的研究  

RESEARCH ON CHROMOSOME ABERRATION OF LYMPHOCYTES IN PERIPHERAL BLOOD OF THE WORKER CONTACTING WITH MIXED BENZENE

作　　者：李秀梅[1] 左惠芬[2] 安金萍[1] 

机构地区：[1]河北工程大学医学院,邯郸056029 [2]邯郸市第一医院检验科

出　　处：《现代预防医学》2009年第19期3635-3636,3639,共3页Modern Preventive Medicine

基　　金：邯郸市科技局基金项目(2000230)

摘　　要：[目的]探讨长期接触混苯对人外周血淋巴细胞染色体结构的影响。[方法]用细胞遗传学方法对78名混苯作业工人(接触组)和78名食品加工厂工人(对照组)进行外周血淋巴细胞染色体畸变、姊妹染色单体互换、微核率进行测定。[结果]接触组染色体畸变、总畸变率及细胞畸变率均高于对照组(P﹤0.01),畸变类型以染色体裂隙和断裂为主,而两者的非整体检出率、姊妹染色体单体互换差异均无统计学意义(P﹥0.05)。接触组无论是微核率和微核细胞率均高于对照组(P﹤0.001)。同时发现,饮酒可能增加混苯的吸收,进而增加对混苯接触者的遗传毒性。[结论]职业性混苯接触可引起人外周血淋巴细胞染色体畸变。[ Objective] To discuss the chromosomal structure of peripheral blood lymphocytes of people contacting with mixed benzene for a long time. [Methods] By the method of cytogenetic, the aberration of peripheral blood lymphocytes, sister chromatid exchange rate and micronucleus rate were detected among 78 mixed-benzene-workers （contacting group） and 78 food processing workers （control group） [Results] chromosomal aberration rate, overall aberration rate and cellular aberration rate in contacting group were higher than those in contrastive group （P 〈 0.01 ）. The major types of aberration were chromosome gap and breakage, but the differences of the aneuploid detection rate and sister chromatid exchange rate between the 2 groups were no statistical significant （ P 〉 0.05）. Micronucleus rate and micronucleus cell rate of contacting group were higher than those of control group （ P 〈 0.001 ）. At the same time. alcohol might be a fact. which would increase absorption of mixed benzene. [ Conclusion ] Occupational contact with mixed benzene induces chromosomal aberration of peripheral blood lymphocytes.

关 键 词：淋巴细胞 混苯 遗传毒性 染色体畸变 

分 类 号：R135.12[医药卫生—劳动卫生]