β2肾上腺素受体基因单核苷酸多态性与河南汉族原发性高血压关系的研究  

STUDY ON THE ASSOCIATION OF SNPS IN β_2-ADRENERGIC RECEPTOR GENE WITH ESSENTIAL HYPERTENSION IN THE HAN POPULATION OF HENAN PROVINCE

作　　者：武红艳[1] 郭利伟[1] 黄艳梅[1] 王克杰[1] 祁英杰[1] 杨宇杰[2] 

机构地区：[1]河南新乡医学院基础医学院,新乡 453000 [2]河南大学民生学院06级生物技术专业

出　　处：《现代预防医学》2009年第19期3755-3757,共3页Modern Preventive Medicine

基　　金：河南省教育厅自然科学研究计划项目(2008A320010);河南省青年骨干教师资助项目(2007)

摘　　要：[目的]探讨β2肾上腺素受体(β2-adrenergic receptor,β2-AR)基因+46(G→A)位和+79(G→C)位单核苷酸多态性(single nucleotide polymorphisms,SNP)与河南汉族原发性高血压(essential hypertension,EH)的相关性。[方法]应用PCR-RFLP的方法,检测汉族高血压患者96例和正常血压者196例的β2-AR基因+46和+79位单核苷酸多态性,并运用卡方分析数据。[结果]两种SNPs基因型分布符合Hardy-Weinberg平衡,+46(G→A)的基因型分布在高血压和对照组的差异无统计学意义(P﹥0.05),+79(G→C)的基因型分布在高血压和对照组中全部是GG,不存在基因多态性。从而得知我国河南汉族人群中β2-AR基因46位点和79位点多态性与高血压病无相关性。但46位点在女性人群中差异有统计学意义(P﹤0.05),与高血压病相关。[结论]46位点可能是河南汉族女性群体原发性高血压遗传的易感性标志之一。[Objective] To study the association of SNPs in regular region of β2-adrenergic receptor gene with essential hypertension of the Han population in ttenan Province. [ Methods ] The samples including 96 patients with hypertension and 196 normal persons were collected from the Han population. C, enotypes of 46 and 79 sites of β2-AR were detertoined by PCRRFLP method. [ Results ] The frequencies of the two SNPs were accord with the Hardy-Weinberg equilibrium in the population. The locus 79 of Beta2-adrenergie receptor gene had no gene polytoorphism. No significant difference was observed in the distribution of genotypes of the SNP at locus 46 between the two groups （P 〉 0.05）. But for the female group, the distribution of genotypes AA. AG. GG of the SNP at locus 46 in hypertension group was significandy different from that in control group （P 〈 0.053. [Conclusion] These results indicate that the locus 46 of Beta2-adrenergic receptor gene may be associated with essential hypertension of the female group.

关 键 词：原发性高血压 Β2肾上腺素受体 单核苷酸多态性 河南汉族 

分 类 号：R544.1[医药卫生—心血管疾病]