层粘连蛋白及其受体的表达与胆囊癌分化、侵袭及转移的相关性研究  

STUDY ON THE CORRELATION OF THE LAMININ AND ITS RECEPTOR EXPRESSION WITH THE DIFFERENTIATION, INVASION AND METASTASIS OF GALLBLADDER CANCER

作　　者：赵欣松 闫立成[2] 刘茜[1] 

机构地区：[1]唐山市工人医院,唐山063000 [2]华北煤炭医学院预防医学系

出　　处：《现代预防医学》2009年第19期3782-3784,3786,共4页Modern Preventive Medicine

摘　　要：[目的]探讨层粘连蛋白(LN)及其受体(LN-R)与胆囊癌恶性程度的关系以及在侵袭、转移过程中的作用,为进一步改善胆囊癌的临床治疗效果提供实验依据。[方法]收集唐山市工人医院胆囊癌手术标本和正常胆囊组织,采用LSAB免疫组化法结合计算机图象分析技术检测胆囊癌组织中LN和LN-R的分布、表达情况。[结果]LN主要存在于基底膜,LN量的减少是基膜成分破坏和胆囊癌恶性表型增高、浸润能力增强的标志。LN-R主要于胆囊癌细胞膜表面和/或胞浆内表达,以前者为主,其表达水平明显高于正常胆囊组织。67KDaLN-R表达水平与肿瘤细胞的侵袭转移能力及恶性表型有关。[结论]胆囊癌组织中LN及LN-R的表达情况与其恶性程度、侵袭转移能力有关。[Objective] To investigate the laminin and its receptor （LN-R） expression in the gallbladder and their correlation with the differentiation, invasion and metastasis of gallbladder cancer. [ Methods] The expression of LN and 67KDa LN-R in the gallbladder cancer and normal tissue were detected by the method of immunohistochemistry. The results were analized by computer image analysis system. [ Results] LN was presented in continuous linear pattern around the trabecular or acinar structures in well-differentiated gallbladder cancer tissue but prasented in discontinuous pattern in poor-differentiated gallbladder cancer tissue. 67KDa LN-R was localized in either cytoplasm or membranous surface of gallbladder cancer cells, especially in poorly-differentiated gallbladder cancer tissue. The expression of 67KDa LN-R was observed in gallbladder cancer with intrahepatic invasion and/or metastasis more than in those without invasion or metastasis （P 〈 0.05）. [ Conclusion] The changes of expression of LN and LN-R might be correlated with invasion, metastasis and prognosis of gallbladder cancer.

关 键 词：胆囊癌 层粘连蛋白 层粘连蛋白受体 免疫组织化学 

分 类 号：R735.8[医药卫生—肿瘤]