rDNA-ITS序列分析鉴定1例念珠菌性甲真菌病病原  

Identification of a pathogen of Candida onychomycosis by rDNA ITS sequence analysis

作　　者：燕勇[1] 朱心强[1] 朱武通 沈志英[2] 王恒辉[2] 陈黎霞[2] 

机构地区：[1]浙江大学医学院营养与食品安全研究所,杭州310058 [2]嘉兴市疾病预防控制中心,浙江嘉兴314050

出　　处：《临床检验杂志》2009年第5期373-375,共3页Chinese Journal of Clinical Laboratory Science

基　　金：浙江省档案局科研项目(NO.2007-9)

摘　　要：目的探讨基于核糖体RNA基因(即rDNA)内转录间隔区(internal transcribed spacer,ITS)多态性的序列分析方法(rD-NA-ITS序列分析)在真菌鉴定中的应用。方法通过PCR扩增与测序的方法测得待检菌株的rDNA-ITS序列,从gene bank获取相似序列,使用BLAST和DNAMAN工具对rDNA-ITS序列进行比对分析,并结合形态学方法进行鉴定。结果该菌株可被准确地鉴定为Candida metapsilosis(原名为近平滑假丝酵母Ⅲ组),与Candida metapsilosis L7685株具有高度同源性(Homology98.6%,Maxident98%)。结论rDNA-ITS序列分析用于真菌鉴定更客观、省时、简便、快速,但也存在一定的应用限制,宜与传统的形态学鉴定方法结合用于真菌鉴定。Objective To investigate the applicability of analyzing the polymorphisms of internal transcribed spacer （ITS） in ribosomal RNA gene （rDNA） in identification of fungi. Methods rDNA 1TS sequences amplified by PCR were analyzed by DNA sequencing. BLAST online tool and DNAMAN software were used to search the most homology sequences in GenBank. Traditional morphological identification was also used. Results Compared with Candida metapsilosis strain L7685 in GenBank, the strain identified in this study had 98.6% homology and 98% maximum identity in the rDNA ITS sequence. The fungus strain was identified as C. metapsilosis （formerly designated as C. parapsilosis Group Ⅲ ）. Conclusions Compared with the morphological method,the rDNA ITS sequence analysis is objective, time-saving, convenient, and fast for fungus identification. However, it can not identify all fungi. The rDNA ITS sequence analysis should be combined with the traditional morphological methods for fungus identification.

关 键 词：RDNA 内转录间隔区(ITS) PCR 念珠菌性甲真菌病 真菌 

分 类 号：R446.5[医药卫生—诊断学]