TNF-α对猪脂肪细胞脂肪分解及其相关基因转录表达的影响  

TNF-α Stimulates Lipolysis in Differentiated Porcine Adipocytes and Downregulated Expression of the Genes Related to Lipolysis

作　　者：郑雪莉[1,2] 李玉成[1] 鞠大鹏[1] 杨公社[1] 

机构地区：[1]西北农林科技大学动物脂肪沉积与肌肉发育实验室,杨凌712100 [2]西北农林科技大学林学院,杨凌712100

出　　处：《畜牧兽医学报》2009年第9期1290-1296,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金："973"国家重点基础研究发展计划(2004CB117506)

摘　　要：为探讨TNF-α对猪脂肪细胞脂肪分解的调控作用及其机制,用1、101、00 ng.mL-13种剂量TNF-α作用体外培养的猪原代脂肪细胞24 h,及10 ng.mL-1TNF-α处理脂肪细胞1、6、12、244、8 h。使用甘油试剂盒,检测细胞甘油的释放量,RT-PCR检测ATGL、TGH-2、HSL、LPL、MGL和perilipinA表达量变化。结果表明,TNF-α以浓度和时间依赖性的方式促进猪原代脂肪细胞甘油的释放(P<0.05);同时下调ATGL、TGH-2、HSL、LPL、MGL和perilipin A mRNA表达量。说明:TNF-α主要通过下调perilipin A的表达,降低其对脂滴的保护作用,增加脂肪分解酶和脂滴的接触,促进了猪脂肪细胞的脂解作用。To study the effect of tumor necrosis factor α（TNF-α） on lipolysis in differentiated porcine adipocytes and its mechanism,the differentiated porcine adipocytes were treated with TNF-α at the concentrations of 1,10,100 ng·mL-1 for 24 hours and the concentration of 10 ng·mL-1 for 0,1,6,12,24 and 48 hours,respectively.Commercial kits were used to measure the glycerol release;Semi-quantitative RT-PCR was used to detect the expression levels of ATGL,TGH-2,HSL,LPL,MGL and perilipin A genes.The results showed that TNF-α promoted the glycerol release in dose and time dependent manners（P〈0.05） in differentiated porcine adipocytes,and down-regulated the mRNA expression levels of ATGL,TGH-2,HSL,LPL,MGL and perilipin A.These results indicated that TNF-α could stimulate lipolysis by down-regulating the expression level of the perilipin A which was thought to modulate the ATGL and HSL to the surface of the fat droplet.

关 键 词：TNF-Α 猪 脂肪细胞 脂肪分解 基因表达 

分 类 号：S828[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]